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General description
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Proteolipid protein 2 (UniProt: Q04941; also known as Differentiation-dependent protein A4, Intestinal membrane A4 protein) is encoded by the PLP2 (Also known as A4) gene (Gene ID: 5355) in human. Proteolipid protein is a multi-pass endoplasmic reticulum membrane protein with four transmembrane domains (aa 25=45; 48-68; 85-105; and 112-132). It is reported to be enriched in colonic mucosa. Its expression follows a gradient along the crypto-villus axis with most abundance in the lower half of the crypt. Its expression is also observed in several areas of the brain. PLP2 is shown to be an oncogenic inducer in several human cancers, including melanoma, osteosarcoma, hepatocellular carcinoma, and lymphoblastic leukemia. Overexpression of PLP2 is also reported in glioma cell lines and suppression of its expression is shown to inhibit glioma cell proliferation and tumorigeneity. PLP2 plays an important role in up-regulation of the PI3K/AKT/mTOR pathway that promotes cell proliferation. It also stimulates MMP2 secretion that can induce cell proliferation, invasion, and metastasis. This ZooMAb® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Chen, Y-H., et al. (2018). Int. J. Mol. Sci. 19(11); 3353; Zhu, H., et al. (2015). Biochem. Biophys. Res. Commun. 459(2); 340-345; Sonoda, Y., et al. (2010). Oncol. Rep. 23(2); 371-376).
We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
Immunogen
KLH-conjugated linear peptide corresponding to 19 amino acids from the C-terminal region of human PLP2.
Application
Quality Control Testing
Evaluated by Western Blotting in PANC1 cell lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected PLP2 in PANC-1 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected PLP2 in lysate from SW480 cells and a 1:10,000 dilution in lysate from MDA-MB-231 cells.
Affinity Binding Assay: A representative lot of this antibody bound PLP2 peptide with at least one thousand-fold (1,000X) higher affinity than with non-specific control peptide.
Immunoprecipitation Analysis: 2 µg from a representative lot immunoprecipitated PLP2 in PANC-1 cell lysate.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 2K5 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects PLP2. It targets an epitope within 19 amino acids from the C-terminal region.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 2K5, recombinant monoclonal |
| description | recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 16.69 kDa,observed mol wt ~17 kDa |
| purified by | using Protein A |
| species reactivity | human |
| packaging | antibody small pack of 25 |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,immunoprecipitation (IP): suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | C-terminal |
| Protein ID accession no. | NP_002659 |
| UniProt accession no. | Q04941 |
| greener alternative category | Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
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