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General description
Research Area: IMMUNO AND CKS
Interleukin-6 Receptor Alpha (IL-6Rα) is a transmembrane protein.
Interleukin-6 (IL-6) mediates its biological effects through the type I IL-6 receptor system that consists of two chains, IL-6Rα and gp130. The IL-6Rα chain is the binding component specific to IL-6; while the gp130 only transmits signals of IL-6 when bound to IL-6Rα. IL-6R has a role in various signaling cascades. The gp130 also can transmit signals from leukemia inhibitory factor (LIF), oncostatin M (OSM), ciliary neurotrophic factor (CNTF), interleukin-11 (IL-11) and cardiotrophin-1 (CT-1) in conjunction with other receptor subunits. The low-affinity binding site for IL-6 is composed of IL-6Rα alone. IL-6Rα is expressed in a wide range of cells including T cells, fibroblasts and macrophages. Soluble IL-6Rα which consists of only the extracellular domain of the IL-6Rα chain, acts as an agonist of IL-6 activity at low concentrations. Recombinant human sIL-6Rα is a 37.6kDa protein consisting of the extracellular domain of the IL-6Rα chain (339 amino acid residues).
Application
sIL-6 Receptor alpha human has been used in cell culture to treat fibroblasts. It has also been used in stimulation experiments to analyze IL-6 trans-signaling.
Biochem/physiol Actions
Interleukin-6 Receptor Alpha (IL-6Rα) plays a dual role in signaling. In classical signaling, it interacts with glycoprotein 130 to promote the anti-inflammatory and regenerative effects of IL-6. In contrast, during trans-signaling, it facilitates the pro-inflammatory effects of IL-6 by allowing cells lacking IL-6Rα to respond to IL-6 in the presence of soluble IL-6Rα. IL-6Rα exists in both soluble and transmembrane forms. The transmembrane form is integrated into the cell membrane of target cells. IL-6 and the IL-6 receptor alpha chain (IL-6R) are expressed by various cell types, including monocytes, lymphocytes, fibroblasts, and endothelial cells.
Physical form
Lyophilized powder.
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Other Notes
LAPRRCPAQE VARGVLTSLP GDSVTLTCPG VEPEDNATVH WVLRKPAAGS HPSRWAGMGR RLLLRSVQLH DSGNYSCYRA GRPAGTVHLL VDVPPEEPQL SCFRKSPLSN VVCEWGPRST PSLTTKAVLL VRKFQNSPAE DFQEPCQYSQ ESQKFSCQLA VPEGDSSFYI VSMCVASSVG SKFSKTQTFQ GCGILQPDPP ANITVTAVAR NPRWLSVTWQ DPHSWNSSFY RLRFELRYRA ERSKTFTTWM VKDLQHHCVI HDAWSGLRHV VQLRAQEEFG QGEWSEWSPE AMGTPWTESR SPPAENEVST PMQALTTNKD DDNILFRDSA NATSLPVQD
| biological source | human |
| recombinant | expressed in CHO cells |
| assay | ≥95% (HPLC),≥95% (SDS-PAGE) |
| form | lyophilized |
| potency | ≤5.0 ng/mL ED50 |
| mol wt | 37.9 kDa |
| packaging | pkg of 20 μg |
| technique(s) | cell culture | mammalian: suitable |
| impurities | <1 EU/μg endotoxin, tested |
| color | white to off-white |
| suitability | suitable for molecular biology |
| UniProt accession no. | P08887 |
| shipped in | wet ice |
| storage temp. | −20°C |
| Gene Information | human ... IL6R(3570) |
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瓶顶过滤器-500ml容量,MF75TM系列，聚苯乙烯外壳，75mmSFCA滤膜，孔径0.2um,适合瓶颈尺寸45mm，12/箱，291-4520，Nalgene，Thermofisher，赛默飞世尔¥ 2856.42
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¥ 1476.00
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¥ 1301.36
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¥ 3852.13
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