蛋白酶 K 来源于林伯氏白色念球菌，5ML，SRE0005-5ML，Sigma

General description

Proteinase K is a stable serine protease with broad substrate specificity. It degrades many proteins in the native state even in the presence of detergents. Proteinase K was isolated from a fungus able to grow on keratin and the enzyme can digest native keratin (hair), hence, the name "Proteinase K". The predominant site of cleavage is the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked alpha amino groups. It is commonly used for its broad specificity.

Application

在分离DNA和RNA的过程中，对核酸酶的蛋白水解失活起作用。
在分离DNA和RNA的过程中，用于核酸酶的蛋白水解失活。
用于去除内毒素结合的阳离子蛋白，如溶菌酶和核糖核酸酶A。
据报道，其可用于分离肝、酵母和绿豆线粒体
用于测定酶在细胞膜上的定位
用于石蜡包埋组织切片的处理，暴露用于抗体标记的抗原结合位点。
用于可传播海绵状脑病(TSE)研究中朊病毒脑组织样本蛋白的消化。

Biochem/physiol Actions

蛋白酶K是一种稳定且具有高活性的丝氨酸蛋白酶。晶体和分子结构研究证据表明该酶属于枯草素家族，具有活性位点催化三联体(Asp³⁹-His⁶⁹-Ser²²⁴)。它在一系列广泛的环境中均稳定：pH，缓冲盐，去污剂（SDS）以及温度。 在0.1-0.5% SDS存在的情况下，蛋白酶K可保持活性，在不损害分离DNA完整性的前提下，可以消化DNA制剂中的多种蛋白质和核酸酶。

Features and Benefits

Features:

1. Qualified Raw Materials: Products that meet the stringent requirements of the Elevated Assurance Program, ensuring compliance with regulatory standards.
2. Comprehensive Documentation: Includes essential documents such as certificates, impurity profiles, and declarations to support audits and risk assessments.
3. M-Clarity^™ Quality Attributes: Products are classified within the MQ300 quality segment or higher, demonstrating superior quality assurance. M-Clarity^™ Program
4. Confirmed Process Controls: Established processes that ensure consistency and reliability in manufacturing.
5. Enhanced Change Notification: Timely updates on any changes to product specifications or processes, ensuring transparency and compliance.
6. Freely Available Dossiers: Access to robust, ready-to-present product dossiers that include:
   1. Site quality assessment
   2. ISO 9001 certificate
   3. Detailed product specifications
   4. Relevant declarations/statements (e.g., animal origin content, BSE/TSE, nitrosamine)

1. Regulatory Compliance: Helps you meet compliance standards effectively, reducing the risk of regulatory issues.
2. Audit Readiness: Instills confidence during audits with comprehensive documentation and confirmed process controls, demonstrating your commitment to high-quality products.
3. Operational Transparency: Supports supply chain transparency, allowing you to track and verify the quality of raw materials.
4. Enhanced Support: Provides exceptional quality-related information and tailored assistance to meet your specific needs.
5. Risk Mitigation: Facilitates thorough risk assessments with detailed documentation, helping you manage potential compliance challenges.
6. Informed Decision-Making: Access to clear and comprehensive product information enables better decision-making and enhances operational efficiency.

Other Notes

在pH 7.5，37 °C 条件下，一单位的酶每分钟将会尿素变性的血红蛋白以产生与1.0 μ摩尔 (181 μg) 酪氨酸等同的颜色（Folin-Ciocalteu试剂提供的颜色）。

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