- 商品介绍
- 规格参数
- 包装参数
Application
Heat-Labile Enterotoxin, B subunit (LTB) from E. coli has been used in enzyme-linked immunosorbent assay (ELISA).
Biochem/physiol Actions
Enterotoxigenic Escherichia coli causes diarrhea through its heat-labile enterotoxin (LT). The LT is a periplasmic protein composed of one A subunit (LTA, 27 kDa) and five non-covalently associated B subunits (LTB, 11.6 kDa each) forming a ring-like pentamer. LTB has high affinity towards the toxin receptor ganglioside GM1, a glycosphingolipid found ubiquitously on the surface of mammalian cells. Ganglioside GM1 facilitates the delivery of the A subunit to the cytosol of the target cell resulting in persistent synthesis of cAMP and subsequently diarrhea. This characteristic makes LTB a good label for microglial cells (due to the enrichment of ganglioside GM1 on their cell surface). In addition, studies made mostly with animal models demonstrated that recombinant LTB could stimulate strong serum and mucosal immune responses against LT. Other studies have indicated that LTB could be used as a potent mucosal adjuvant.
Heat-Labile Enterotoxin, B subunit (LTB) is a good label for microglial cells. In addition, recombinant LTB stimulates strong serum and mucosal immune responses against heat-labile enterotoxin (LT). Other studies have indicated that LTB could be used as a potent mucosal adjuvant.
Features and Benefits
This compound is a featured product for Cyclic Nucleotide research. Click here to discover more featured Cyclic Nucleotide products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.
Physical form
Lyophilized from a solution containing Heat-Labile Enterotoxin, B subunit in 0.05 M Tris buffer, pH 7.5, 0.2 M NaCl, 3 mM NaN3, and 1 mM sodium EDTA.
Analysis Note
The activity is measured by ELISA using ganglioside GM1-coated plates (Cat. No. G7641). LTB at various concentrations is incubated on the ganglioside GM1 coated wells, followed by anti-cholera toxin antibody (Cat. No. C3062), and peroxidase-labeled goat anti-rabbit IgG (Cat.No. A0545) as the secondary antibody. Binding saturation of 50% may be achieved with = 0.12 μg/mL of LTB.
| recombinant | expressed in Pichia pastoris |
| Quality Level | 200 |
| assay | >90% (SDS-PAGE) |
| form | lyophilized powder |
| shipped in | wet ice |
| storage temp. | 2-8°C |
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¥ 2290.21
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¥ 2013.69
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¥ 1866.29
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¥ 238.17
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¥ 3812.28
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¥ 4404.38
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¥ 2288.54
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¥ 4614.63
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¥ 3662.39
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