E-Cadherin/Fc Chimera from mouse，50UG，E2153-50UG，Sigma 供应商、价格、厂家、报价、规格参数

产品介绍 Product Description

General description

E (epithelial)-cadherin is a type I classical cadherin, which exists as a dimer. It has an ectodomain made of five extracellular cadherin-like (EC) domains, connected by conserved linker regions, which are calcium-binding in nature. It spans the membrane once, and contains a cytoplasmic region. It is a glycoprotein, and in humans has a molecular weight of 120kDa. It binds to catenin and cytoskeletal protein with its C-terminal domain.
Extracellular domain of mouse E-Cadherin (amino acids 1-709) fused to the carboxy-terminal 6X histidine tagged Fc region of human IgG1.

Application

E-Cadherin/Fc Chimera from mouse has been used in adhesion assays.

Biochem/physiol Actions

E (epithelial)-cadherin is involved in hemophilic cell-cell adhesion in a calcium-dependent manner. It forms adheren junctions through its ectodomains, to maintain physical contacts between adjacent cells. It plays essential roles in developmental processes, tissue homeostasis and multiple diseases. In humans, this protein might be involved in the pathophysiology of ulcerative colitis. In Crohn′s diseased patients, mutations in this gene lead to a truncated or mislocalized protein product. Epithelial-mesenchymal transition (EMT) and mesenchymal-epithelial transition (MET) are the underlying processes of tumorigenesis and embryogenesis, and these involve the control of E-cadherin expression.

Physical form

Lyophilized from 0.2 μm filtered solution in 20mM MES, 300mM NaCl, 1mM CaCl2.

技术参数 Specifications

biological source	mouse
Quality Level	100
recombinant	expressed in NSO cells
sterility	sterile; sterile-filtered
assay	>90% (SDS-PAGE)
form	lyophilized powder
mol wt	monomer calculated mol wt 88.2 kDa, ~120 kDa by SDS-PAGE (reducing),calculated mol wt 88.2 kDa
packaging	pkg of 50 μg
technique(s)	cell culture \| mammalian: suitable
impurities	endotoxin, tested
UniProt accession no.	P09803
shipped in	ambient
storage temp.	−20°C
Gene Information	mouse ... Cdh1(12550)

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