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General description
AT-rich interactive domain-containing protein 1A (UniProt: O14497; also known as ARID domain-containing protein 1A, B120, BRG1-associated factor 250, BAF250, BRG1-associated factor 250a, BAF250A, Osa homolog 1, hOSA1, SWI-like protein, SWI/SNF complex protein p270, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily F member 1, hELD) is encoded by the ARID1A (also known as BAF250, BAF250A, C1orf4, OSA1, SMARCF1) gene (Gene ID: 8289) in human. ARID1A is a member of a large family of proteins that contain a characteristic ARID domain of approximately 100 amino acids that binds DNA fragments rich in adenine-thymine (AT). It is a component of SWI/SNF chromatin complexes and is highly expressed in spleen, thymus, prostate, testis, ovary, small intestine, and colon. At lower level it is also expressed in several other tissues. It is involved in transcriptional activation and repression of select genes by chromatin remodeling. It changes chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. ARID1A is shown to be critical for embryonic stem cell pluripotency and their differentiation into mesoderm-derived cardiomyocytes and adipocytes. It is also a component of the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. Nuclear ARID1A is shown to be more unstable than its cytoplasmic counterpart, as it degrades rapidly, by the ubiquitin-proteasome system of the nucleus. It is normally detected during the G0-G1 phase of the cell cycle and its levels decrease during the G2-S phase. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 22 amino acids from the internal region of human ARID domain-containing protein 1A (ARID1A).
Application
Quality Control Testing
Evaluated by Western Blotting in HeLa cell lysate..
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ARID1A in HeLa cell lysate.
Tested applications
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected ARID1A in HeLa, A431, HUVEC and NIH 3T3 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected ARID1A in human colon tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound ARID1A with a KD of 4.3 x 10-8 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biochem/physiol Actions
Clone 1C3 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects ARID domain-containing protein 1A (ARID1A). It targets an epitope within 22 amino acids from the internal region. May detect all three isoforms.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 1C3, recombinant monoclonal |
| description | recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 242.05 kDa,observed mol wt ~250 kDa |
| purified by | using Protein A |
| species reactivity | mouse, human |
| species reactivity (predicted by homology) | rat, monkey, canine, bovine |
| packaging | antibody small pack of 25 μL |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,immunocytochemistry: suitable,immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | Internal |
| Protein ID accession no. | NP_006006 |
| UniProt accession no. | O14497 |
| greener alternative category | Aligned, |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... ARID1A(8289) |
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