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General description
Affinity purified VHH single domain antibody lyophilized from PBS, pH7.7
The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It′s been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. Alpaca derived anti-SARS-CoV-2 Spike RBD VHH single domain antibody (molecular weight: 13.6kDa), expressed in E. coli under conditions free from animal derived components.It has a 6His tag at its C-terminal.
Single-domain antibody (sdAb), also called nano-antibody, is the variable region isolated from the antigen-binding variable domain (VHH) of camelid heavy-chain-only antibody. It lacks light chains and a first constant domain(CH1). This configuration confers a number of unique advantages over conventional monoclonal antibodies. The small size of just 15 kDa (2~4 nm), single-domain antibody is the smallest antigen-binding fragment that can easily penetrate tissues, allowing them to bind epitopes that are inacessible to conventional antibodies. These single-domain antibodies offer superior chemical and thermal stability and increased solubility than conventional antibodies. All our antibodies are tested to ensure the highest level of specificity, sensitivity and reproducibility.
Immunogen
Recombinant SARS-CoV-2 spike RBD (Arg319-Phe541) protein
Application
ELISA
Biochem/physiol Actions
SARS-CoV-2 Spike RBD
Packaging
50 µg per vial / 100 µg per vial
Preparation Note
Reconstitute the lyophilized antibody with sterile deionized water or with 50% glycerol (ACS grade or better) to a stock solution of 1 mg/mL. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.
Stable for 1 year at 4°C from date of receipt.
Other Notes
Storage at 4°C; Do not freeze.
| biological source | alpaca |
| Quality Level | 100 |
| recombinant | expressed in E. coli |
| conjugate | unconjugated |
| antibody form | affinity purified VHH antibody |
| clone | SS95, recombinant monoclonal |
| assay | >95% (SDS-PAGE) |
| form | lyophilized solid |
| mol wt | 13.6 kDa, ~15-18 kDa (SDS PAGE under reducing condition) |
| purified by | immobilized metal affinity chromatography (IMAC) |
| species reactivity | (SARS-CoV-2) |
| concentration | 1 mg/mL |
| technique(s) | ELISA: 1:3000-1:10000 |
| color | colorless |
| pH | ( in PBS, pH 7.4) |
| isotype | VHH |
| Protein ID accession no. | QOS45029.1 |
| UniProt accession no. | P0DTC2 |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | SARS coronavirus ... S(43740568) |
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