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General description
Intercellular adhesion molecule 1 (UniProt: P05362; also known as ICAM-1, Major group rhinovirus receptor, CD54) is encoded by the ICAM1 gene (Gene ID: 3383) in human. CD54 is a member of the immunoglobulin supergene family with five extracellular Ig like domains that are involved in cell-cell and cell-matrix adhesion. It is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-27), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 28-481), a transmembrane domain (aa 481-503), and a cytoplasmic domain (aa 504-532). It is reported to be constitutively expressed in several hematopoietic and non-hematopoietic cells, including fibroblasts, endothelial cell, leukocytes, and epithelial cells. Its expression can be upregulated by several pro-inflammatory cytokines. Stronger expression of CD54 has been observed in neoplastic cells from stem cell derived neoplasms. The endothelial expression of CD54 is showed to be higher in atherosclerotic and transplant-associated atherosclerotic tissue. Its adhesive actions are mediated by binding to CD11a/CD18 (LFA-1) and CD11b/CD18 (Mac-1). Its adhesive interactions are shown to be essential for the transendothelial migration of leukocytes out of the blood vessels and into tissues. It also plays a role in the activation of T cells where it serves as a co-activation signal. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Lawson, C., and Wolf, S (2009). Pharmacol Rep. 61(1); 22-32; Roebuck, KA., and Finnegan, A. (1999). J. Leukoc. Biol. 66(6); 876-888; Maio, M., and Del Vecchio, L (1992). Leuk. Lymphoma. 8(2-2); 23-33).
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 18 amino acids from the N-terminal, extracellular domain of human CD54 (ICAM-1).
Application
Quality Control Testing
Evaluated by Western Blotting in Ramos cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CD54 in Ramos cell lysate.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected CD54 in lysates from human liver tissue and MCF-7 cells.
Flow Cytometry Analysis: 1 μg from a representative lot detected CD54 in one million human peripheral blood mononuclear cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CD54 in human lung tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound ICAM-1/CD54 with a KD of 2.0 x 10-8 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biochem/physiol Actions
Clone 2C15 is a Rabbit recombinant monoclonal antibody that specifically detects CD54. It targets an epitope within 18 amino acids from the N-terminal, extracellular domain.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 2C15, recombinant monoclonal |
| description | recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 57.82 kDa,observed mol wt ~100 kDa |
| purified by | using Protein A |
| species reactivity | human |
| species reactivity (predicted by homology) | monkey |
| packaging | antibody small pack of 25 μL |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,flow cytometry: suitable,immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | Extracellular domain |
| Protein ID accession no. | NP_000192 |
| UniProt accession no. | P05362 |
| greener alternative category | Aligned, |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
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