- 商品介绍
- 规格参数
- 包装参数
General description
This recombinant human calprotectin is a heterodimer composed of tagged versions of both S100A8 and S100A9, where a 6xHis Sumo tag is added to the N-terminus of S100A8 and, a 6XHis tag is added to the N-terminus of S100A9. This tagged version was found to have enhanced stability as compared to untagged versions of this protein. Removal of the tags can be achieved using HRV-3C protease (SAE0045 or SAE0110).
Application
Fecal Calprotectin is often used as a biomarker to identify inflammation in gastrointestinal disease1. This product is a recombinant version of the human protein found endogenously.
Biochem/physiol Actions
Calprotectin is a calcium and zinc binding protein that belongs to the S100 family. It is a heterodimer protein composed of S100A8 and S100A9.1 The protein is expressed in myeloid cells and in circulating monocytes and neutrophils, where it accounts for 60% of the cytosolic protein content. Calprotectin was found to play major roles in inflammation and the immune response by activation of NF-kB pathway via TLR4 and AGER,2 and its antimicrobial activity possibly through chelating of Zn.3 High concentrations of calprotectin are found and serve as a biomarker in rheumatoid, cystic fibrosis, inflammatory bowel disease and in Crohn′s disease.4 A meta-analysis also identified calprotectin as a potential biomarker for COVID-19.5
Physical form
The product is lyophilized from a sterile 0.2 µm filtered solution containing 12.5 mM Tris-Cl (pH 8.0), 150 mM NaCl, and 1 mM DTT.
Preparation Note
Reconstitute lyophilized vial in DMEM or in PBS to a concentration up to 500 µg/mL. Spin down to collect material. Reconstituted product should be stored in working aliquots at –20 °C. Do not store in a frost-free freezer. Avoid freeze-thaw cycles.
| Quality Level | 200 |
| assay | ≥90% (SDS-PAGE) |
| form | lyophilized powder |
| shipped in | wet ice |
| storage temp. | −20°C |
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