- 商品介绍
- 规格参数
- 包装参数
General description
Phosphatidylserine (PS) is a phospholipid (1,2-diacyl-sn-glycerol-(3)-L-phosphoserine) found in the membrane of all plants and animals. It is highly concentrated in the brain and may make up about 10% of the lipid content of neurons. Neuronal PS is reported to play a role in maintaining nerve cell integrity, enhancing neurotransmitter signal efficiency, and improving nerve cell signal transmission. It is required for healthy nerve cell membranes and myelin. It is usually found on the inner side of the plasma membrane, but during early-stage apoptosis, it translocates to the outer leaflet of plasma membrane. FITC-labeled Annexin V can bind to the exposed PS, providing the basis of most annexin V-based apoptosis assays. Anti-Phosphatidylserine can be is used to detect the translocation of the membrane PS in cells undergoing apoptosis. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, Affinity™, reproducibility, and stability over conventional monoclonals. (Ref.: Glade, MJ., and Smith, K. (2015). Nutrition. 31(6); 781-786; Lee, SH., et al. (2013). Cell Death Diffren. 20(1); 64-76).
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
Liposomes containing 70% phosphatidylserine and 30% phosphatidylglycerol.
Application
Quality Control Testing
Evaluated by Immunocytochemistry in Jurkat cells treated with Staurosporine.
Immunocytochemistry Analysis: A 1:1,000 dilution of this antibody detected Phosphatidylserine in Jurkat cells treated with staurosporine (2 µg/ml; 8 h).
Tested Applications
Flow Cytometry Analysis: 1 µg from a representative lot detected Phosphatidylserine in one million Jurkat cells treated with Staurosporine (2 µg/ml; 8 h).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 1H6 is a ZooMAb® mouse recombinant monoclonal antibody that specifically detects Phosphatidylserine.
Physical form
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
Affinity is a trademark of Mine Safety Appliances Co.
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 1H6, recombinant monoclonal |
| description | recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® learn more |
| form | lyophilized |
| purified by | using protein G |
| species reactivity | human |
| species reactivity (predicted by homology) | all |
| packaging | antibody small pack of 25 μL |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | flow cytometry: suitable,immunocytochemistry: suitable |
| isotype | IgG |
| greener alternative category | Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
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