miRCURY LNA Premium miRNA Mimic (5) - 3' Biotin，339178，Qiagen，凯杰

miRCURY LNA miRNA Mimics are designed to simulate naturally occurring mature miRNAs. Introducing an miRNA mimic into cells increases the proportion of RNA-induced silencing complexes (RISC) containing the guide strand miRNA, enabling assessment of the phenotypic consequences of increased activity and discovery of new miRNA functions. miRCURY LNA miRNA Mimics have an innovative design that includes two short, LNA-enhanced complimentary strands that prevent any miRNA-like activity associated with the passenger strands, so you can be sure that phenotypes observed using these mimics are due to increased activity of the mimicked miRNA. Need a quote for your research project or would you like to discuss your project with our specialist team? Just contact us!

• Third generation of highly potent, mature miRNA mimics with unique triple RNA strand design
• No miRNA-star activity from bisected LNA-enhanced passenger strand
• miRNA strand sequence matches miRBase annotation
• Available with fluorescent label to assess transfection efficiency
• Available with biotinylated miRNA strand to isolate targets by RNA pull-down

miRNA mimics simulate the natural functions of endogenous miRNAs and are primarily used in gain-of-function studies by assessing the biological consequences of increasing miRNA activity. The effect of increasing the cellular content of an miRNA using miRNA mimics can be studied in numerous ways, including cellular assays to monitor cell proliferation, cell differentiation or apoptosis. The effect on gene expression can also be measured at the mRNA or protein level of putative miRNA targets.
miRNA mimics are also frequently used for validating miRNA targets in combination with miRNA inhibitors and target site blockers. Typically, plasmid-based assays are used in which the 3' UTR of the mRNA under investigation has been cloned downstream of a reporter gene. Introducing the mimic into cells harboring the reporter plasmid will reduce reporter gene expression, while miRNA inhibitors and target site blockers masking the 3' UTR miRNA binding site will cause derepression.
Biotinylated miRNA mimics are highly effective tools for identification of miRNA targets in RNA pull-down experiments. Recent advances with this experimental approach are transforming our understanding of miRNA biology and have revealed that non-canonical miRNA–mRNA interactions, which are ignored by target prediction tools, are frequent and lead to target repression. The principle of the technique is shown in Perfect miRNA strand-specific activity with miRNA mimics. Biotinylated mimics are significantly less potent than non-modified mimics.