HotStarTaq Master Mix Kit (250 U)，203443，Qiagen，凯杰

3 x 0.85 ml HotStarTaq Master Mix (contains 250 units HotStarTaq DNA Polymerase, PCR Buffer with 3 mM MgCl2, and 400 µM of each dNTP)and 2 x 1.7 ml RNase-Free Water.

高特异性扩增，用于多种PCR分析

高PCR特异性，无需优化

操作简单，室温下进行反应体系的构建

即用型预混液，减少移液步骤

HotStarTaq Master Mix包含HotStarTaq DNA Polymerase、可将优化需求最小化的独特的QIAGEN PCR Buffer、以及dNTPs。预混液中提供各种所需组分，减少移液步骤，降低污染风险，同时提高通量和可重复性。

每一批HotStarTaq Master Mix Kit都经过全方位的质量控制测试，包括：严格的PCR特异性和可重复性分析，即使低拷贝的靶分子也可扩增。通过检测，HotStarTaq DNA Polymerase优于其它供应商提供的试剂盒，确保高度特异性和在热启动PCR中的卓越表现。

该试剂盒提供的新型PCR缓冲液使得在多种PCR条件下都维持高特异性，无需优化。

 高度特异性配合简单的操作使HotStarTaq Master Mix Kit适用于复杂的基因组或cDNA模板、多重引物对、从珍贵样本或低拷贝靶分子中抽提的模板（参见"Highly sensitive single-cell PCR"）。该试剂盒还适用于大量样本的扩增，诸如基因筛查等项目。

HotStarTaq DNA Polymerase特性

浓度：5 units/µl
重组酶：是
底物类似物：dNTP、ddNTP、dUTP、biotin-11-dUTP、DIG-11-dUTP、fluorescent-dNTP/ddNTP
延伸速度：72°C条件下2–4 kb/min
酶半衰期：97°C条件下10 min，94°C条件下60 min
扩增效率：≥105倍
5'–>3'外切酶活性：是
额外添加A：是
3'–>5' 外切酶活性：否
核酸酶污染：否
蛋白酶污染：否
RNases污染：否
自启活性：否  

Higher specificity with different primer–template systems.

Three different primer-template systems were amplified under the same conditions with either Taq DNA polymerase from Supplier R (R) or with HotStarTaq DNA Polymerase (H). System 1: A 1.1 kb fragment of a D-IgI homolog was amplified from human genomic DNA. System 2: A 296 bp fragment from the chromosomal region correlated with X-linked juvenile retinoschisis was amplified from human genomic DNA. System 3: A 214 bp fragment of the β-actin gene was amplified from cDNA synthesized from total RNA. M: markers. Note: Data are shown for HotStarTaq DNA Polymerase; identical sensitivity and specificity were obtained with HotStarTaq Plus DNA Polymerase.

Increased specificity of primer annealing.

Tolerance to variable temperatures and magnesium concentrations.

[A] PCR amplification at the indicated annealing temperatures using QIAGEN PCR Buffer and Taq DNA Polymerase (QIAGEN). The same PCR was performed in parallel using PCR buffer and Taq DNA polymerase from another supplier (Supplier AII). The single-copy human cystic fibrosis gene was amplified. M: markers. [B] Tolerance to Variable Magnesium Concentration. PCR amplification at the indicated Mg2+ concentrations using QIAGEN PCR Buffer and Taq DNA Polymerase (QIAGEN). The same PCR was performed in parallel using PCR buffer and Taq DNA polymerase from another supplier (Supplier AII). The single-copy human prion protein gene was amplified. M: markers.

Higher specificity with different primer–template systems.

Three different primer-template systems were amplified under the same conditions with either Taq DNA polymerase from Supplier R (R) or with HotStarTaq DNA Polymerase (H). System 1: A 1.1 kb fragment of a D-IgI homolog was amplified from human genomic DNA. System 2: A 296 bp fragment from the chromosomal region correlated with X-linked juvenile retinoschisis was amplified from human genomic DNA. System 3: A 214 bp fragment of the β-actin gene was amplified from cDNA synthesized from total RNA. M: markers. Note: Data are shown for HotStarTaq DNA Polymerase; identical sensitivity and specificity were obtained with HotStarTaq Plus DNA Polymerase.

Effect of hot start on RT-PCR performance.

A 1.1 kb fragment of the human interleukin 1 receptor (type II) gene was amplified from cDNA. Amplification reactions were prepared in triplicate using Taq DNA polymerase and buffer from Supplier L(No hot start); antibody-mediated hot start using enzyme and buffer from Supplier L (Antibody-mediated); and HotStarTaq DNA Polymerase and PCR Buffer from QIAGEN (HotStarTaq). M: markers.

Highly sensitive single-cell PCR.

A 500 bp fragment of the murine p53 gene was amplified from single cells isolated by flow cytometry, and directly sorted into individual PCR tubes. Reactions were prepared in parallel using HotStarTaq DNA Polymerase and PCR Buffer from QIAGEN (HotStarTaq), a hot-start enzyme and buffer from Supplier AII (Hot-start enzyme), or antibody-mediated hot start and buffer from Supplier L (Antibody-mediated). M: markers.

Superior performance.

A 497 bp fragment was amplified from 50 copies of an HIV-pol-gene construct which had been added to 1 µg human genomic DNA. Different hot-start enzymes were employed: HotStarTaq DNA Polymerase from QIAGEN (HotStarTaq); hot-start enzyme from Supplier AII (Hot-start enzyme); Taq-antibody mixture from Supplier L (Antibody-mediated); enzyme without hot start from Supplier R (No hot start). Specific PCR product is indicated by the arrow. Equal volumes of the reaction were analyzed on a 2% agarose gel. M: markers.

HotStarTaq procedure.

The HotStarTaq procedure is fast and easy for maximum convenience.

HotStarTaq Master Mix Kit适合多种应用，包括各种富有挑战性的研究，如各种扩增应用：

复杂的基因模板

复杂的cDNA模板（如RT-PCR）

低拷贝的靶分子（如单细胞PCR）

多重引物对反应