QIAwave RNA Plus Mini Kit (50)，74634，Qiagen，凯杰

The QIAwave RNA Plus Mini Kit is a more eco-friendly version of our standard RNeasy Plus Mini Kit, combining high-quality RNA purification with effective gDNA removal using unique gDNA Eliminator columns. Total RNA of high purity and yield is obtained from a wide range of cultured cells and easy-to-lyse tissues.  The QIAwave RNA Plus Mini Kit uses up to 47% less plastic and up to 42% less cardboard than our standard kit and offers Waste Tubes made from 100% post-consumer recycled plastic that you can reuse throughout the procedure. QIAwave buffers also come as concentrates, reducing the amount of plastic by up to 90% per bottle. To save paper, there are no printed protocols in the kit. Instead, you can download the protocols you need from the resources list or by simply scanning the QR code inside the box lid. So, while the kit packaging and components of our QIAwave RNA Plus Mini Kit may look different, it’s as easy to use as the RNeasy Plus Mini Kit, and the chemistry and performance are identical. Please be aware that you will need sterile glass bottles to store the reconstituted buffers. In partnership with My Green Lab, we've assessed the environmental impact of this kit. My Green Lab ACT labels evaluate and score products on several sustainability criteria: Manufacturing Responsible chemical management Sustainable content within products and packaging materials Disposal of the packaging at the end of life Products are scored from 1 to 10 except for energy and water consumption, which are scored 1 point per kWh or gallon, respectively. A low score means a lower impact (see figures "QIAwave RNA Plus Mini Kit ACT label US  50 / 250 , EU  50 / 250 and UK  50 / 250 ").  

• Phenol-free high-quality total RNA isolation in minutes using fast and simple extraction protocols
• RNA quality, yields and performance identical to the RNeasy Plus Mini Kit
• Efficient gDNA removal with unique gDNA Eliminator columns (no need for DNase)
• Up to 47% less plastic and up to 42% less cardboard compared to the RNeasy Plus Mini Kits
• Reusable Waste Tubes made from 100% post-consumer recycled plastic
• Buffer concentrates that use up to 90% less plastic than our standard buffer bottles

Features	Specifications
Applications	RNA-seq, quantitative real-time RT-PCR, end-point RT-PCR, Northern dot and slot blotting, microarray analysis
Elution volume	30-50 µL
Technology	Silica technology
Format	Spin column
Integrated removal of genomic DNA	Yes
Sample type	Cells, tissues
Sample amount	107 cells or 30 mg tissue
Processing	Manual or automated on QIAcube Connect
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein	Total RNA
Time per run	25 minutes

RNA purified using the QIAwave RNA  Plus Mini Kit is ideally suited for downstream applications that are sensitive to low amounts of DNA contamination, such as RNA-seq and quantitative real-time RT-PCR. The purified RNA can also be used in other applications.