- 商品介绍
- 规格参数
- 包装参数
QuantiTect PCR Kits (qPCR kits) enable sensitive quantification of gDNA and cDNA targets by real-time PCR and two-step RT-PCR using sequence-specific probes or SYBR Green I detection. The real-time PCR kits also allow reliable quantification of up to 5 gDNA or cDNA targets in a single tube by multiplex, real-time PCR or two-step RT-PCR. The combination of a hot start and a unique PCR buffer system in the ready-to-use master mix ensures highly sensitive qPCR on any real-time cycler without the need for optimization. The dNTP mix includes dUTP, allowing optional treatment with UNG. For convenience, the master mixes in QuantiTect PCR Kits can be stored at 2–8°C.
Two kit formats are available for multiplex PCR using sequence-specific probes: the QuantiTect Multiplex PCR Kit for cyclers that require ROX dye for fluorescence normalization, and the QuantiTect Multiplex PCR NoROX Kit for all other cyclers.
Highly sensitive detection of low-copy targets
qPCR using sequence-specific probes or SYBR Green
Accurate quantification over several logs of template
No need to optimize reaction and cycling conditions
Detection of up to 5 targets in the same tube
Features | QuantiTect SYBR Green PCR Kits | QuantiTect Probe PCR Kits | QuantiTect Multiplex PCR Kits |
Applications | Real-time quantification of genomic DNA or cDNA targets | Real-time quantification of genomic DNA or cDNA targets | Real-time quantification of genomic DNA or cDNA targets |
Reaction type | PCR and two-step RT-PCR | PCR and two-step RT-PCR | Multiplex PCR and multiplex two-step RT-PCR |
Real-time or endpoint | Real-time | Real-time | Real-time |
Sample/target type | DNA, cDNA | DNA, cDNA | DNA, cDNA |
Single or multiplex | Single | Single | Multiplex |
SYBR Green I or sequence-specific probes | SYBR Green I | Sequence-specific probes | Sequence-specific probes |
Thermal cycler | All real-time cyclers (e.g., LightCycler, Rotor-Gene, ABI) | Most real-time cyclers (e.g., LightCycler, Rotor-Gene, ABI) | Most real-time cyclers (e.g., LightCycler, Rotor-Gene, ABI) |
With or without ROX | With ROX | With ROX | With or without ROX |
QuantiTect PCR Kits can be used for gene expression analysis of cDNA or quantification of gDNA on any real-time cycler. This includes instruments from Applied Biosystems, Bio-Rad, Cepheid, Eppendorf, Roche and Agilent. For the Rotor-Gene Q and other Rotor-Gene cyclers, we recommend using the Rotor-Gene SYBR Green PCR Kit, Rotor-Gene Probe PCR Kit or Rotor-Gene Multiplex PCR Kit, which have been specially developed for fast cycling on these instruments.
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