AllPrep DNA/mRNA Nano (12)，80272，Qiagen，凯杰

Collect genetic and transcriptomic information from CTCs and rare cells, with minimal cell quantities, by isolating gDNA and mRNA from the same sample, at the same time. The AllPrep DNA/mRNA Nano Kit uses a two-step magnetic-bead-based method that lets you maximize mRNA capture and isolate high-molecular-weight DNA for multiomics studies.

• Simultaneous gDNA and mRNA isolation
• Maximal yields from minimal cell quantities
• High-molecular-weight, sequencing-ready gDNA
• Messenger RNA suitable for single-cell RNA-seq application
• Magnetic-bead-based system

Features	Specifications
Applications	Transcriptome and genome profiling, real-time PCR, cDNA synthesis, NGS, RNA seq
Elution volume	25 µl
Format	Magnetic-bead-based system
Main sample type	Low-biomass samples (rare cells, CTCs, fetal cells, stem cells, T-cells)
Processing	Manual (magnetic rack)
Product use	Purification of full-length messenger RNA and high-molecular-weight genomic DNA
Sample amount	Down to 1 cell
Technology	Magnetic beads
Time per run or per prep	75 min per prep

Genomic DNA purified with the AllPrep DNA/mRNA procedure has a high molecular weight of up to 100 kb. This purified DNA is suitable for any downstream application, such as PCR, multiplex PCR, dPCR, pyrosequencing or NGS.

The purified mRNA is of high quality and can be used in any application, such as RT-PCR, real-time RT-PCR, or cDNA generation, including the AdnaTest procedure for molecular characterization of CTCs.