- 商品介绍
- 规格参数
- 包装参数
产品介绍
Product Description
QIAseq Stranded RNA Library Kits provide a superior method for generating Illumina compatible RNA-seq libraries from total RNA or mRNA enriched samples. For applications such as gene expression, fusion gene or mutation detection, QIAseq Stranded mRNA Select Kits include an optimized mRNA enrichment protocol with all the reagents and components required to build high-quality RNA-seq libraries. QIAseq Stranded RNA Library Kits use a unique protocol, which does not require actinomycin D to retain strand specificity or dUTP to ensure stranded library construction, thereby ensuring highly sensitive detection of low-expression RNA molecules with increased complexity and transcript coverage. QIAseq Stranded RNA Library Kits use CleanStart HiFi PCR Mastermix to ensure high-fidelity amplification of libraries and to protect from PCR contamination. CleanStart HiFi PCR Mastermix is included in each kit or can be used separately with other library preparation methods. Analyze stranded RNA-seq data with ease using the GeneGlobe-integrated RNA-seq Analysis Portal – an intuitive, web-based data analysis solution created for biologists and included with QIAseq Stranded RNA Library Kits. Want to try this solution for the first time? Request a quote for a trial kit. For one-step, rRNA and globin mRNA removal in just 20 minutes, use QIAseq FastSelect –rRNA HMR Kits.
产品特点
Features
- Stranded, high-quality RNA-seq libraries for transcriptome analysis
- Only 100–5000 ng of total RNA or 1–100 ng of poly-A+ enriched RNA required
- Includes QIAseq Beads and sample adapter plates
- Compatible with fresh, as well as FFPE samples
- Includes access to the RNA-seq Analysis Portal for human, mouse and rat samples
产品应用
Applications
QIAseq Stranded RNA Library Kits generate RNA-seq libraries from low RNA amounts of various origins and species. The resulting highly complex and strand-specific NGS libraries, in combination with the decontaminating properties of the CleanStart PCR Mix, allow the analysis of even low-abundance transcripts with high sensitivity. The streamlined, 4–5 hour protocol allows the generation of NGS libraries, library QC measurements and the start of an NGS sequencing run in just one working day.
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