QIAseq Single Cell RNA Lib Kit UDIA (96)，180705，Qiagen，凯杰

The QIAseq Single Cell RNA Library Kit UDI is an end-to-end library preparation solution for RNA-seq from single cells, cell populations or small quantities of RNA. The kit includes all reagents required for cell lysis, reverse transcription, cDNA amplification and PCR-free NGS library preparation, including QIAseq Beads for reaction cleanup and QIAseq Unique Dual-Index Y-Adapter Kits for sample indexing. This kit produces high-quality NGS libraries and generates a tube of excess amplified cDNA that can be stored for follow-up experiments. The kit is highly suited for transcript discovery and differential gene expression from single eukaryotic cells, enriched cell populations and RNA-seq from limited samples, including viral RNA. The QIAseq Single Cell RNA Library Kit UDI allows researchers to focus on mRNAs, explore the transcriptome (mRNAs and lncRNAs) or enrich for small RNA genomes (such as viruses) from low-input RNA samples. This versatile kit enables multiple RNA-seq applications by choosing from several different protocols included with the kit. Analyze RNA-seq data with ease using the GeneGlobe-integrated RNA-seq Analysis Portal – an intuitive, web-based data analysis solution created for biologists and included with this QIAseq Kit.

• Start with single cells, cell populations or enrich for small RNA genomes or viruses
• Choose to focus on mRNAs, or explore the transcriptome
• REPLI-g RNA amplification and PCR-free protocol reduces bias and provides greater reproducibility
• Unique dual indices (included) ensure high-quality data from NovaSeq and patterned flow cells
• Sequencing-ready libraries from isolated single cells in just 5.5 hours

• Gene expression profiling
• Transcript detection of mRNA/lncRNAs
• Viral genomics and transcriptomics