- 商品介绍
- 规格参数
- 包装参数
AllPrep DNA/RNA Kits enable simultaneous purification of genomic DNA and total RNA from each cell or tissue sample. Since there is no need to divide each sample into two for separate purification procedures, maximum yields of DNA and RNA can be achieved. The purified DNA and RNA are eluted separately.
The AllPrep DNA/RNA Micro and Mini Kits can be automated on the QIAcube Connect. The AllPrep DNA/RNA 96 Kit is well suited for high-throughput purification of genomic DNA and total RNA from up to 96 samples.
High-quality DNA and RNA from the same sample
Maximal yields of DNA and RNA from precious samples
Rapid purification with short, streamlined protocol
Streamlined protocols for 96-well plates
Ready-to-use DNA and RNA for any downstream analysis
Comparison of AllPrep DNA/RNA Kits
Features | AllPrep DNA/RNA Micro Kit | AllPrep DNA/RNA Mini Kit | AllPrep DNA/RNA 96 Kit |
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Applications | PCR, RT-PCR, real-time PCR, cDNA synthesis, microarray, blotting | PCR, real-time PCR, microarray, blotting | PCR, real-time PCR, microarray, blotting |
Elution volume | DNA: 30 µl; RNA: 10 µl | 100 µl | 50–100 µl |
Format | Spin column | Spin column | 96-well plate |
Main sample type | Cells, tissue | Cells, tissue | Cultured cells, easy-to-lyse tissue |
Processing | Manual (centrifugation) | Manual (centrifugation) | Manual (centrifugation and/or vacuum) |
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | Genomic DNA and total RNA | Genomic DNA and total RNA | Total RNA, DNA, miRNA (optional) |
Sample amount | 5 x 105 cells or 5 mg tissue | 1 x 107 cells or 30 mg tissue | Up to 2 x 106 cells/up to 10 mg tissue |
Technology | Silica technology | Silica technology | Silica technology |
Time per run or per prep | 35 min | 35 min | 60 min |
Yield | Varies | Varies | Varies |
The purified genomic DNA has an average length of 15–30 kb, depending on homogenization conditions, and is suited for any application, including next-generation sequencing, Southern-, dot-, and slot-blot analyses; and PCR and multiplex PCR.
The purified total RNA can be used in any application, such as RNA-seq RT-PCR and real-time RT-PCR; differential display; cDNA synthesis; northern-, dot-, and slot-blot analyses; and microarrays.
A supplementary protocol allows optional purification of protein. The purified protein is denatured and can be used in applications such as SDS-PAGE, western blotting, and 2D gel electrophoresis.
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