AllPrep DNA/RNA Mini Kit (50),80204,Qiagen,凯杰

销售价: ¥ 8890.00 / 件
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订货号 1RS5923
品牌型号 Qiagen 80204
货期 45个工作日
最小订货量 1件
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产品介绍 Product Description

AllPrep DNA/RNA Kits enable simultaneous purification of genomic DNA and total RNA from each cell or tissue sample. Since there is no need to divide each sample into two for separate purification procedures, maximum yields of DNA and RNA can be achieved. The purified DNA and RNA are eluted separately.

The AllPrep DNA/RNA Micro and Mini Kits can be automated on the QIAcube Connect. The AllPrep DNA/RNA 96 Kit is well suited for high-throughput purification of genomic DNA and total RNA from up to 96 samples.


产品特点 Features
  • High-quality DNA and RNA from the same sample

  • Maximal yields of DNA and RNA from precious samples

  • Rapid purification with short, streamlined protocol

  • Streamlined protocols for 96-well plates

  • Ready-to-use DNA and RNA for any downstream analysis


技术参数 Specifications

Comparison of AllPrep DNA/RNA Kits

FeaturesAllPrep DNA/RNA Micro KitAllPrep DNA/RNA Mini KitAllPrep DNA/RNA 96 Kit
ApplicationsPCR, RT-PCR, real-time PCR,
cDNA synthesis, microarray, blotting
PCR, real-time PCR, microarray,
blotting
PCR, real-time PCR, microarray,
blotting
Elution volumeDNA: 30 µl; RNA: 10 µl100 µl50–100 µl
FormatSpin columnSpin column96-well plate
Main sample typeCells, tissueCells, tissueCultured cells, easy-to-lyse tissue
ProcessingManual (centrifugation)Manual (centrifugation)Manual (centrifugation and/or vacuum)
Purification of total RNA, miRNA,
poly A+ mRNA, DNA or protein
Genomic DNA and total RNAGenomic DNA and total RNATotal RNA, DNA, miRNA (optional)
Sample amount5 x 105 cells or 5 mg tissue1 x 107 cells or 30 mg tissueUp to 2 x 106 cells/up to 10 mg tissue
TechnologySilica technologySilica technologySilica technology
Time per run or per prep35 min35 min60 min
YieldVariesVariesVaries


产品应用 Applications

The purified genomic DNA has an average length of 15–30 kb, depending on homogenization conditions, and is suited for any application, including next-generation sequencing, Southern-, dot-, and slot-blot analyses; and PCR and multiplex PCR.

The purified total RNA can be used in any application, such as RNA-seq RT-PCR and real-time RT-PCR; differential display; cDNA synthesis; northern-, dot-, and slot-blot analyses; and microarrays.

A supplementary protocol allows optional purification of protein. The purified protein is denatured and can be used in applications such as SDS-PAGE, western blotting, and 2D gel electrophoresis.


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