QIAprep&amp Buffer AB (16.4 ml)，221517，Qiagen，凯杰

The QIAprep& Viral RNA UM Kit is an innovative liquid-based approach to viral RNA epidemiology. The kit is optimized for ultrafast sample preparation and sensitive real-time PCR detection of enveloped RNA viruses such as coronaviruses from human samples (nasal, oro- and nasopharyngeal swabs) collected in non-fixation transport media like UTM, VTM, PBS, 0.9% NaCl, Virocult and eSwab. The kit is also compatible with neat saliva and gargle samples. Fewer and simpler workflow steps accelerate the turnaround time and increase testing frequency while decreasing plastic usage, cost and hands-on time. The method can be seamlessly integrated into automated workflows with standard lab equipment at all throughput needs. The QIAprep& Viral RNA QIAsymphony Kit can be used for automated sample preparation and assay set up on the QIAsymphony SP/AS. The QIAprep& Viral RNA QIAsymphony Kit requires the installation of a custom protocol available from QIAGEN Services. The QIAprep& Viral RNA UM Kit can be used in conjunction with the SARS-CoV-2 N1+N2 Assay Kit. Want to try the QIAprep& Viral RNA UM Kit for the first time? Register to start your trial. For labs that need to track currently relevant mutations of the virus, wet-lab tested LNA-based qPCR genotyping assays have been designed to detect the variants of concern (VOCs). The assay performance in terms of specificity and sensitivity has been verified using the QIAprep& Viral RNA UM Kit. View the assays here.

• Sample preparation and real-time PCR-based virus detection integrated into one single kit
• Time to result in under 1 hour
• End-to-end liquid-based workflow for complete automation on liquid handlers
• Compatibility with any assay and real-time PCR cycler
• 4-plex (RT)-PCR capability for detection of up to 4 targets, including viral targets and/or controls
• Includes two controls (inhibition and sampling) for reliable interpretation of results
• Two-phase hot-start procedure and stable RT included in the master mix for stability and specificity

The QIAprep& Viral RNA UM Kit simplifies and accelerates real-time PCR-based detection of enveloped RNA viruses such as SARS-CoV-2 coronavirus from human samples for epidemiological research purposes.