REPLI-g Advanced DNA Single Cell Kit(96),150365,Qiagen,凯杰

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订货号 1NT4220
品牌型号 Qiagen 150365
货期 45个工作日
最小订货量 1件
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产品介绍 Product Description
DNA sequence analysis and genotyping of biological samples using next-generation sequencing (NGS) platforms is often limited by the amount of available sample. The REPLI-g Advanced DNA Single Cell Kit has been specifically optimized for use in sensitive microarray and NGS applications, providing high uniformity, with minimal allelic dropout, when starting with single cells or purified genomic DNA. Buffers and reagents undergo a unique, controlled decontamination procedure to minimize contaminating DNA and ensure highly reliable results. Accurate amplification of genomes with negligible sequence bias and minimal genomic dropouts is achieved with innovative Multiple Displacement Amplification (MDA) technology.


产品特点 Features
  • WGA with complete genome coverage from single eukaryotic cells or 1 ng genomic DNA
  • Simple 3-step protocol requires only 2 hours and 20 minutes, start to finish
  • Consistent yields of up to 25–35 µg DNA from 1 eukaryotic cell
  • Includes cryo-protect reagent to minimize DNA damage during single cell capture, storage or shipping



产品应用 Applications
Downstream applications and instrumentation
Application Instrumentation
Whole genome sequencing Next-generation sequencing platforms*
Exome sequencing
SNP genotyping arrays Array platforms*
Array CGH
qPCR/PCR technologies Real-time PCR/PCR cyclers*
Sanger sequencing Capillary sequencers*
Pyrosequencing PyroMark (QIAGEN)
Learn more about our single cell products by visiting our Single Cell Resource.

References

1. Hosono, S. et al. (2003) Unbiased whole-genome amplification directly from clinical samples. Genome Res. 13, 954.


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