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General description
Protein spindly, otherwise known as the coiled-coil domain-containing protein 99 (CCDC99), is a phosphoprotein that regulates mitosis. It interacts with the Rod/ZW10/Zwilch complex, and is involved in recruiting, docking, and alignment of motor protein dynein at mitotic kinetochores. Spindly may also be involved in checkpoint signaling and microtubule attachment.
~70 kDa observed. This protein is known to exist in two isoforms at 70 kDa and 56 kDa An uncharacterized band may be observed at ~38 kDa in some cell lysates.
Immunogen
GST-tagged recombinant protein corresponding to human Protein Spindly.
Application
Detect Protein Spindly using this Anti-Protein Spindly Antibody, clone 9D10.2 validated for use in ICC, Western Blotting.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Protein Spindly in HeLa and NIH/3T3.
Research Category
Cell Structure
Research Sub Category
Cytoskeleton
Physical form
Format: Purified
Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
HEK293 cell lysate
Evaluated by Western Blot in HEK293 cell lysate.
Western Blot Analysis: 0.1 µg/mL of this antibody detected Protein Spindly in 10 µg of HEK293 cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 9D10.2, monoclonal |
| species reactivity | human, mouse |
| technique(s) | immunocytochemistry: suitable,western blot: suitable |
| isotype | IgMκ |
| NCBI accession no. | NP_060255 |
| UniProt accession no. | Q96EA4 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... SPDL1(54908) |
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