- 商品介绍
- 规格参数
- 包装参数
产品介绍
Product Description
QuantiFast Pathogen +IC Kits使用序列特异性探针,可灵敏、快速的对病原体核酸进行real-time PCR或一步法RT-PCR检测。该试剂盒含有检测4个用户定义的病原体核酸(如:病毒、细菌、真菌等)所需的试剂和内参,可对阴性结果进行正确解读,提高了检测灵敏度。该试剂盒有两种规格。QuantiFast Pathogen RT-PCR +IC Kit含有RNA内参模板和内参引物/探针对,用于检测病毒RNA。QuantiFast Pathogen PCR +IC Kit含有DNA内参模板和内参引物/探针对,用于检测病毒、细菌或真菌DNA。这两种试剂盒均提供分装在两个管中的不同浓度的ROX染料,因此适用于多种real-time PCR仪。为便于使用,预混液可存放在2–8°C。
产品特点
Features
- 同时检测目标病原体和内参
- 5x预混液具有更高的灵敏度,且起始样本量更大
- 正确解读阴性信号,检测灵敏度更高
- 清晰检测微弱的阳性信号
- 快速的通用型实验方案适用于标准和快速PCR仪
技术参数
Specifications
Features | Specifications |
---|---|
Applications | Pathogen Detection: Real-time PCR of viral, bacterial or fungal DNA (QuantiFast Pathogen PCR +IC Kit) or one-step RT-PCR for detection of viral RNA (QuantiFast Pathogen RT-PCR +IC Kit) |
Sample/target type | QuantiFast Pathogen PCR +IC Kit: viral, bacterial or fungal DNA; QuantiFast Pathogen RT-PCR +IC Kit: viral RNA |
Single or multiplex | Duplex |
Reaction type | Real-time PCR or one-step RT-PCR including of an internal control (IC) |
Real-time or endpoint | Real-time |
SYBR Green I or sequence-specific probes | Sequence-specific probes |
Thermal cycler | For most standard and fast real-time cylcers compatible with duplex PCR/RT-PCR, e.g. Rotor-Gene Q or cyclers from Agilent, Applied Biosystems, BioRad, Roche |
With or without ROX | Master Mix is provided without ROX dye, but 2 separate ROX solutions are included: High-ROX Dye Solution for use with ABI cyclers except ABI 7500, ROX Dye Solution (low ROX conc.) for use with ABI 7500 and other suppliers |
产品应用
Applications
QuantiFast Pathogen +IC Kits利用序列特异性探针进行灵敏的real-time PCR或一步法RT-PCR,检测病原体DNA和/或RNA,以及内参。该试剂盒适用于多种real-time PCR仪,包括QIAGEN、Applied Biosystems、Bio-Rad、Roche和Agilent的仪器。
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