- 商品介绍
- 规格参数
- 包装参数
产品介绍
Product Description
QuantiTect Whole Transcriptome Kit对有限的RNA样品进行预扩增和逆转录，获得高产量cDNA，用于无限次real-time PCR基因表达分析。该试剂盒包含全转录扩增所需的全部酶和缓冲液。对Phi29聚合酶创新的修饰确保从低至1 ng RNA中制备多至40 μg cDNA。该聚合酶独特的加工过程可制备高度统一的cDNA，确保通过real-time PCR进行可靠的基因表达分析。
产品特点
Features
- 高cDNA产量用于无限次分析和长期保存
- 均一扩增所有cDNA和所有转录本区段
- 经优化的试剂，快速简单的操作流程
技术参数
Specifications
| Features | Specifications |
|---|---|
| Amplification | Whole total RNA |
| Starting material | Purified total RNA |
| Reaction time | 5 hours or 11 hours |
| Applications | Real-time PCR, gene expression analysis |
| Minimal pipetting volume needed | 1 µl |
| Samples per run (throughput) | Mid |
| Technology | Reverse transcription followed by ligation and multiple displacement amplification (MDA) |
| Yield | 10 µg or 40 µg cDNA |
| Maximum input volume | 5 µl total RNA |
| Starting amount of total RNA | >10 ng purified total RNA |
| Reaction volume | 50 µl |
产品应用
Applications
QuantiTect Whole Transcriptome Kit制备的cDNA可用于QuantiFast或Rotor-Gene Kits进行的real-time PCR分析，且可以储存用于以后的分析，但不适用于微阵列分析。QIAGEN提供REPLI-g Kits and Service，用于小量或珍贵样品的全基因组扩增。扩增高度一致，具有最小的序列偏好，可制备适合于基因分型和Comparative Genome Hybridization等应用的DNA。
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