规格
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Enzyme:
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DNase |
Shipping Condition:
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Wet Ice |
储存
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DNase I is supplied as 1 vial containing 20,000 units at a concentration of 50-375 U/µl. Store in freezer (-5°C to -30°C). |
DNase I (Deoxyribonuclease I) digests single- and double-stranded DNA to oligodeoxyribonucleotides containing a 5' phosphate. Ribonuclease has been reduced to non-detectable levels.
Applications
DNase I is suitable for removing DNA from protein preparations, nick translating DNA, and generating random fragments for dideoxy sequencing.
NOTE: for removing DNA from RNA preparations, use Amplification Grade DNase I (Cat. No. 18068-015).
Source
DNase I is purified from bovine pancreas.
Specific activity
The specific activity of DNase I is typically in the range of 10,000–25,000 units/mg.