规格
|
Cleavage:
|
TEV Protease Recognition Site |
Cloning Method:
|
Restriction Enzyme ⁄ MCS |
Product Line:
|
Bac-to-Bac™ |
Promoter:
|
Polyhedrin |
Vector:
|
pFastBac |
Protein Tag:
|
His Tag (6x) |
储存
|
The Bac-to-Bac® HT Vector Kit includes 10 µg of pFastBac™ HT vector and pFastBac HT-CAT control vector.
Store the vectors at -20°C. All components are guaranteed stable for 6 months when properly stored. |
The Bac-to-Bac® HT Vector is designed for use as part of the Bac-to-Bac® Baculovirus Expression System (Cat. No. 10359-016) for the expression and purification of histidine-tagged recombinant proteins in Sf9, Sf21, or High Five™ Cells following bacmid generation in E. coli. The pFastBac™ HT vector offers the following features:
• Strong polyhedrin promoter for protein expression
• Three reading frames for simplified cloning
• N-terminal 6xHis tag for simple purification of recombinant fusion proteins
• TEV protease cleavage site for removal of the histidine tag following protein purification