- 商品介绍
- 规格参数
- 包装参数
规格 |
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Assay: | Reporter Enzyme |
Compatible Cells: | Mammalian Cells |
Label Type: | Enzyme Labeled |
Product Line: | Pierce™ |
Substrate: | Proprietary |
Substrate Properties: | Chemical Substrate |
Target Enzyme: | Beta-Galactosidase |
Technique: | Absorbance |
Description: | Pierce Mammalian beta-Galactosidase Assay Kit |
Format: | 96-well plate |
For Use With (Equipment): | Microplate Reader |
Substrate Type: | Beta-Gal Substrate |
Detection Method: | Colorimetric |
Quantity: | 75 mL Kit |
储存 |
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Sufficient For: 250 microplate wells of adherent cells • Pierce beta-Galactosidase Assay Reagent, 25 mL • M-PER Mammalian Protein Extraction Reagent, 25 mL • Stop Solution, 25 mL Store the β-Galactosidase Assay Reagent at -20°C. Store all other components at room temperature. |
描述 |
The Thermo Scientific Mammalian β-Galactosidase Assay Reagent is a component of the Mammalian β-Galactosidase Assay Kit, which provides a simple colorimetric method for lysing cultured mammalian cells and measuring beta-Gal activity.
The Assay Reagent and Kit comprise a simple and versatile assay system for measuring β-galactosidase activity. Mammalian cells grown on a plate may be lysed and tested for β-galactosidase activity by adding a single, all-in-one lysis-and-assay solution. Alternatively, the included Thermo Scientific M-PER Mammalian Protein Extraction Reagent may be used to prepare lysate from washed adherent or pelleted cells, and then a portion of the lysate used to quantitate the β-Gal activity. With M-PER Reagent, there is no need to scrape cells or to perform freeze-thaw cycles. The remaining lysates prepared with M-PER Reagent may be used for applications such as reporter assays (luciferase, β-galactosidase and chloramphenical acetyltransferase), protein kinase assays (PKA, PKC, and Tyrosine Kinase), immunoassays (Western blot, ELISA, RIA) and protein purification.
Features of the Thermo Scientific Mammalian β-Galactosidase Assay Kit (assay reagent and stop solution):
• Choose stand-alone assay reagent or complete kit for cell lysis and detection
• Colorimetric assay easily measured in a plate reader or spectrophotometer (405nm)
• Adaptable to 96-well microplates or tube assay formats
• Perform homogeneous screening assays or measure activity secondarily after cell lysis
• Also compatiible with prepared lysates from other species such as bacteria
Related Products
Mammalian beta-Galactosidase Assay Kit
beta-Galactosidase Assay Stop Solution
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