AMPK (A2/B2/G2) 10 ug,PV6250,Invitrogen

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订货号 6CZ8294
品牌型号 Invitrogen PV6250
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最小订货量 1套
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产品介绍 Product Description
规格
Assay Entry: Biochemical binding/kinetic
Kinase Group: Serine/Threonine Kinase
Species: Human
Target Entry: AMPK (A2/B2/G2)
Molecular Weight: 63.1/ 31.4/ 59.3 kDa
Gene Alias: Subunit A2: PRKAA2, AMPK, AMPK2, PRKAA Subunit B2: PRKAB2, MGC61468 Subunit G2: PRKAG2, AAKG, CMH6, WPWS, AAKG2, H91620p
Validated Application: Kinase Assay
Protein Tag: His Tag
储存
Storage Buffer: 50 mM sodium phosphate pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.2 mM DTT, 25% glycerol

Store at -68 to -85°C. Aliquot at first use to avoid multiple freeze-thaws.
描述

AMP-activated protein kinase (AMPK) exhibits a key role as a master regulator of cellular energy homeostasis. AMPK exists as a heterotrimeric complex composed of a catalytic α subunit and regulatory beta; and gamma; subunits. Binding of AMP to the gamma; subunit allosterically activates the complex. AMPK is activated in response to stresses that deplete cellular ATP (low glucose, hypoxia and ischemia) and via signaling pathways in response to adiponectin, leptin and CAMKK beta;. Recombinant full-length human AMPK (combination of A2/B2/G2 subunits) was expressed by baculovirus in Sf9 insect cells using C-terminal His tags.

• Validated in LanthaScreen® Kinase Binding Assay
• Protocols available for simple use and optimized performance
• Multiple pack sizes available for convenient use

Related Product:
LanthaScreen® Eu Kinase Binding Assay

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