- 商品介绍
- 规格参数
- 包装参数
规格 |
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Assay Entry: | Biochemical binding/kinetic |
Kinase Group: | Serine/Threonine Kinase |
Species: | Human |
Target Entry: | AMPK (A2/B2/G2) |
Molecular Weight: | 63.1/ 31.4/ 59.3 kDa |
Gene Alias: | Subunit A2: PRKAA2, AMPK, AMPK2, PRKAA Subunit B2: PRKAB2, MGC61468 Subunit G2: PRKAG2, AAKG, CMH6, WPWS, AAKG2, H91620p |
Validated Application: | Kinase Assay |
Protein Tag: | His Tag |
储存 |
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Storage Buffer: 50 mM sodium phosphate pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.2 mM DTT, 25% glycerol Store at -68 to -85°C. Aliquot at first use to avoid multiple freeze-thaws. |
描述 |
AMP-activated protein kinase (AMPK) exhibits a key role as a master regulator of cellular energy homeostasis. AMPK exists as a heterotrimeric complex composed of a catalytic α subunit and regulatory beta; and gamma; subunits. Binding of AMP to the gamma; subunit allosterically activates the complex. AMPK is activated in response to stresses that deplete cellular ATP (low glucose, hypoxia and ischemia) and via signaling pathways in response to adiponectin, leptin and CAMKK beta;. Recombinant full-length human AMPK (combination of A2/B2/G2 subunits) was expressed by baculovirus in Sf9 insect cells using C-terminal His tags.
• Validated in LanthaScreen® Kinase Binding Assay
• Protocols available for simple use and optimized performance
• Multiple pack sizes available for convenient use
Related Product:
LanthaScreen® Eu Kinase Binding Assay
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