- 商品介绍
- 规格参数
- 包装参数
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规格 |
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| Bacterial or Yeast Strain: | DH10B |
| Blue/White Screening: | Yes |
| Cloning Methylated DNA: | Yes |
| Cloning Unstable DNA: | Not Suitable for cloning unstable DNA |
| Competent Cell Type: | Electrocompetent |
| F'episome (to make ssDNA): | Lacks F' Episome |
| Format: | Tube(s) |
| High-throughput Compatibility: | Not High-throughput Compatible (Manual) |
| Improves Plasmid Quality: | Yes |
| Plasmid: | May be used for plasmids > 100 kb |
| Preparing Unmethylated DNA: | Not Suitable for preparing unmethylated DNA |
| Product Line: | DH10B, Electrocomp™ |
| Propagating ccdB Vectors: | Not for ccdB vector propagation |
| Reduces Recombination: | Yes |
| Shipping Condition: | Dry Ice |
| Species: | E. coli |
| T1 Phage - Resistant (tonA): | Yes |
| Transformation Efficiency: | > 3x10^10 |
| Transformation Efficiency Level: | High Efficiency (> 10^9 cfu⁄µg) |
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储存 |
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Contains: • MegaX DH10B T1R Electrocomp C: 5 vials, 100 µl each • pUC19 DNA (10 pg/µl): 1 vial, 50 µl • Recovery Medium: 3 bottles, 10 ml each Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store Recovery Medium at 4°C . |
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描述 |
MegaX DH10B T1R Electrocomp Cells are the highest-efficiency electrocompetent cells available. Improved production processes now guarantee a three-fold greater number of colonies per transformation (>3 x 1010 cfu/µg of pUC DNA), making the MegaX DH10B T1R cells ideal for highly demanding cloning and library construction. They have the same genotype as the widely used DH10B T1R strain, including tonA, which prevents infection by T1 and T5 lytic bacteriophages, and safeguards your valuable clones and libraries.
Using MegaX DH10B T1R cells
MegaX DH10B T1R cells provide highly efficient transformation of methylated DNA, since they lack the E. coli K12 restriction systems (mcrA Δ(mrr-hsdRMS-mcrBC)). The Φ80lacZΔM15 marker provides α-complementation of the β-galactosidase gene, allowing blue/white screening on agar plates containing X-gal or Bluo-gal.
Genotype: F- mcrA Δ(mrr-hsdRMS-mcrBC), Φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG tonA
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| 重量(kg) |
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¥ 1048.84
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¥ 673.51
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¥ 807.83
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¥ 3683.17
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询价
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¥ 1958.88
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¥ 1155.55
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