规格
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Assay Entry:
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Cell-based terbium modification activity (transient) |
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Cell State:
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Cells Not Included |
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Human Protein Class:
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Cell Signalling |
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Product Line:
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LanthaScreen® |
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Readout:
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End Point |
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Reporter Gene:
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GFP (EmGFP) |
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System Type:
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LanthaScreen® |
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Target Entry:
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Histone H3 |
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Technique:
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TR-FRET |
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Detection Method:
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Fluorescent |
储存
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Kit contains Terbium-labeled anti-Histone H3 antibody, BacMam Histone H3 Virus, and 6X Lysis Buffer. Store as indicated in handling documentation. |
The combination of baculovirus-mediated gene delivery (BacMam) with LanthaScreen® Cellular Assay technology enables a platform for the analysis of specific posttranslational modifications of histones. BacMam provides a convenient genetic delivery tool for a GFP-Histone H3 fusion protein in the cell line of interest. This kit describes an HTS-compatible cellular immunoassay measuring acetylation of GFP-Histone H3 at Lys9.