CELLROX GREEN FLOW ASSAY KIT KIT，C10492，Applied Biosystems 供应商、价格、厂家、报价、规格参数

产品介绍 Product Description

规格
Compatible Cells:	Mammalian Cells, Eukaryotic Cells
Detection Method:	Fluorescent
Excitation/Emission:	CellROX® green: 508⁄525, SYTOX® red: 640⁄658
Flow Cytometer Laser Lines:	633/635, 488
For Use With (Equipment):	Flow Cytometer
Format:	Tube(s)
Label or Dye:	CellROX® Green Reagent
No. of Reactions:	100
Product Line:	CellROX®
Shipping Condition:	Wet Ice
Solubility:	DMSO (Dimethylsulfoxide)

储存
Contains 1 vial CellROX® Green Reagent (25 µL), 1 vial SYTOX® red dead cell stain (100 µL), 2 vials N-acetylcysteine (10 mg per vial), 1 vial tert-butyl hydroperoxide (50 µL, 70% solution in water), and 1 vial DMSO (200 µL). Store kit at -5 to -30°C, protected from light. CellROX® reagent is air sensitive.

描述

The CellROX™ Green Flow Cytometry Assay Kit enables the flow cytometric detection of reactive oxygen species (ROS) in live cells. The kit includes the novel, fluorogenic CellROX™ Green Reagent, as well as SYTOX™ Red Dead Cell Stain, N-acetyl cysteine (an antioxidant, for negative control), and tert-butyl hydroperoxide solution (TBHP, an inducer of ROS).

View a selection guide for all CellROX® Reagents and Kits.

The CellROX™ Green Flow Cytometry Assay Kit features:

• Fluorogenic probe formulated for flow cytometry that is oxidized in the presence of ROS
• Multicolor compatibility—minimal overlap with fluorophores excited by other laser lines, allowing easy multiplexing with other reagents
• Simple protocol—cells can be stained in complete media or other appropriate buffer, no need for serum free media

The CellROX™ Green Detection Reagent is cell-permeable and non-fluorescent or very weakly fluorescent while in the reduced state. Upon oxidation, the reagent binds DNA and exhibits a strong fluorogenic signal that has absorption/emission maxima of 508/525 nm and remains localized to the nucleus and cytoplasm. When used together with the included SYTOX™ Red Dead Cell Stain, oxidatively stressed and nonstressed cells are reliably distinguished from dead cells by flow cytometry.

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