规格
|
Cell Line:
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293E (HEK), 293 (HEK), Expi293F™, FreeStyle™ 293-F Cells, 293F |
Classification:
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Animal Origin-Free, Chemically Defined, Protein-Free, Serum-Free |
Culture Environment:
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CO2 |
Culture Type:
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Suspension Cell Culture |
Endotoxin Level:
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< 1 EU/ml |
Form:
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Liquid |
Glucose:
|
High Glucose |
Glutamine:
|
GlutaMAX™-I |
HEPES Buffer:
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No HEPES |
Included Antibiotics:
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No antibiotics |
Inorganic Salts:
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Calcium, Magnesium, Sodium Phosphate |
Osmolality:
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275-295 mOsm/kg |
Phenol Red Indicator:
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No Phenol Red |
Product Line:
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Expi293™ |
Purity or Quality Grade:
|
Cell Culture Grade |
Serum Level:
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Serum-Free |
Shipping Condition:
|
Room Temperature |
Sodium Bicarbonate Buffer:
|
Sodium Bicarbonate |
Sodium Pyruvate Additive:
|
Sodium Pyruvate |
Supplements:
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No Hypoxanthine, No Thymidine, Insulin-Free, No Vitamin A, No 2-Mercaptoethanol, Dextrose, Pyridoxine HCl |
Cell Type:
|
Kidney (Embryonic) |
Concentration:
|
1 X |
Format:
|
Bottle(s) |
Sterility:
|
Sterile |
储存
|
Store in the dark at +2°C to +8°C |
Gibco Expi293 Expression Medium is a chemically defined, serum-free, protein-free medium for growth and transfection of suspension-adapted human embryonic kidney (HEK) 293 cells. It is specifically designed as a core component to support high density culture of Expi293F cells in the Expi293 Expression System for scalable transient protein expression.
• Supports growth of suspension 293 cultures to densities of over 1.5 x 10^7 cells/mL
• Transfection compatible serum-free suspension culture medium enables transfection efficiencies of greater than 70% using ExpiFectamine transfection reagent
• Enables sustained, high level expression of high density transiently transfected cultures, achieving yields of up to 1 gram per liter of recombinant protein
• Supports growth and transfection of 293 cells in culture formats of less than 1 mL in multiwell plates to greater than 10L in disposable bioreactors
• Does not contain phenol red
Expi293 Expression Medium is formulated with GlutaMAX-I reagent. It is ready to use, with no supplementation required. The chemically defined formulation results in high reproducibility and lot-to-lot reliability. Expi293 Expression Medium contains no human or animal-origin components. The medium is not recommended for adherent cell cultures.
Product use
Customers using Expi293 Expression Medium in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).