OREGON GREEN 488 PHALL 300 U，O7466，Invitrogen

Oregon Green® 448 phalloidin is a high-affinity F-actin probe conjugated to green fluorescent Oregon Green® 488 dye.

• Selectively stains F-actin
• Outstanding fluorescence performance
• Excitation/Emission: 496/520 nm
• Superior to antibody staining
• Optimal for fixed and permeabilized samples
• pH insensitive under physiological conditions

Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides 'death cap' mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while Oregon Green® 488 provides green fluorescence with brightness superior to fluorescein. Oregon Green® 488 is a fluorinated analog of fluorescein with greater photostability and a lower pKa, making its fluorescence essentially pH insensitive in the physiological pH range. Demonstrating very little nonspecific staining, Oregon Green® 488 phalloidin allows high-contrast discrimination of actin staining.

Use in Multiple Applications
Oregon Green® 448 phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Oregon Green® 448 phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot® nanocrystals, and Alexa Fluor® conjugates including secondary antibodies.

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links
• Read about labeling F-Actin with phallotoxins.
• Create a Cellular Masterpiece with Alexa Fluor® Phalloidin using the Cell Paint Tool.