规格
|
Bioassay:
|
Proliferation of HUVECs (Human Umbilical Vein Endothelial Cells) |
Endotoxin Level:
|
< 0.1 ng/µg |
Form:
|
Lyophilized |
Mass:
|
10 µg |
Product Line:
|
Gibco® |
Protein Form:
|
Recombinant, Ligand |
Purity or Quality Grade:
|
Carrier-Free |
Research Category:
|
Oncology, Angiogenesis, Stem Cell Research, Differentiation, Inflammation |
Shipping Condition:
|
Wet Ice |
Species:
|
Human |
Specific Activity:
|
1.0 x 106 - 1.7 x 105 units⁄mg |
Molecular Weight:
|
40 kDa |
Purification Method:
|
Sequential Chromatography |
Purity:
|
>95 % |
Quantity:
|
10μg |
Protein Subtype:
|
VEGF (Vascular Endothelial Growth Factor) |
Gene Alias:
|
VEGF |
Validated Application:
|
Cell Culture |
Protein Family:
|
Growth Factors & Receptors |
Expression System:
|
Mammalian |
Protein Length:
|
165 aa |
Biological Activity:
|
ED50 range = 1-6 ng⁄mL |
储存
|
Store this lyophilized preparation at 2 to 8°C. Upon reconstitution, apportion into working aliquots and store at ≤ –20°C. Avoid repeated freeze⁄thaw cycles. |
Recombinant Human Vascular Endothelial Cell Growth Factor (VEGF) is a bioactive protein intended for use in cell culture applications. VEGF exerts angiogenic, mitogenic and vascular permeability-enhancing activities specific for endothelial cells. VEGF has also been shown to be chemotactic for monocytes and osteoblasts.
• High purity—no interference from other proteins or contaminants
• High biological activity—more results with less protein
• Proven compatibility—Gibco® proteins bioassayed with Gibco® media
High Purity Recombinant Bioactive Protein
Recombinant Human Vascular Endothelial Cell Growth Factor is one of our more than 250 available Gibco® recombinant proteins. To help ensure Gibco® growth factors are of the highest quality, each protein is analyzed for purity along with structural homogeneity to ensure a biologically active protein.
High Biological Activity
In-house activity testing of Gibco® Recombinant Proteins includes cell proliferation, cytotoxicity, chemotaxis, calcium flux, secondary cytokine up-regulation, induction of surface antigen expression, antiviral, and protease assays. Recombinant Human Vascular Endothelial Cell Growth Factor (VEGF) specific activity is determined by the dose dependent proliferation of human umbilical vein cells (HUVEC).
Proven Compatibility
Gibco® proteins are bioassayed with Gibco® media. Since 1962, Gibco® has been the consistent provider of high quality media, reagents, and sera for reliable cell culture.
See our selection of recombinant Gibco® growth factors.
For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.