规格
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For Use With (Application):
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Real-Time Quantitative PCR (qPCR), Transfection, Cloning, Sequencing, In Vitro Transcription, Transformation, Nucleic Acid Labeling, PCR |
No. of Reactions:
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25 Preps |
Sample Type:
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Bacteria |
High-throughput Compatibility:
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Not High-throughput Compatible (Manual) |
Prep Scale:
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> 200 µg (Large-Scale) Plasmid DNA |
Purification Target:
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Plasmid DNA |
Product Line:
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GeneJET |
Thermo Scientific GeneJET Plasmid Maxiprep Kit, sufficient for 25 preps, utilizes silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 750 µg of high copy plasmid DNA that can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription, and transfection of robust cell lines.
Highlights
• Efficient—high yields up to 750 µg of high quality plasmid DNA from 250 mL of bacterial culture
• High concentration of DNA purified—> 400 ng/ µL
• Convenient—spin column format, centrifuge or vacuum protocols
• Fast—procedure takes 60 minutes, considerably faster than using gravity flow columns
Applications
• Isolated plasmid DNA is suitable for all conventional molecular biology procedures, including:
• Transfection of robust cell lines
• FastDigest restriction enzyme digestion
• Automated fluorescent sequencing
• PCR
• Cloning
• Transformation
• In vitro transcription