- 商品介绍
- 规格参数
- 包装参数
规格 |
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Final Product Type: | Total RNA |
For Use With (Application): | Laser Capture Microdissection (LCM), Reverse Transcriptase PCR (RT-PCR), Northern Blotting |
Isolation Technology: | Silica Spin Column |
Sample Type: | Cell Cultures, Laser Capture Microdissection (LCM), Cultured Cells |
Elution Volume Max: | 20 µl |
High-throughput Compatibility: | Not High-throughput Compatible (Manual) |
Purification Target: | Total RNA |
Scale: | Micro |
Product Line: | Ambion™, RNAqueous® |
储存 |
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• 50 Micro Filter Cartridge Assembly (room temperature) • 50 Micro Elution Tubes (room temperature) • 15 ml Wash Solution 1 Concentrate (room temperature) • 28 ml Wash Solution 2/3 concentrate (4°C) • 25 Lysis Solution (4 °C) • 200 µl LCM Additive (-20°C) • 55 µl DNase 2 U/µl (-20°C) • 600 µl 10X DNase I Buffer (-20°C) • 150 µl DNase Inactivation Reagent (-20°C) • 5 ml Elution Solution (-20°C, 4°C, room temperature) |
描述 |
The RNAqueous®-Micro Kit is used for phenol-free total RNA isolation from micro-sized samples (e.g., from <500,000 cultured cells, from few as 10 laser capture microdissected cells, or from ≤10 mg of tissue) using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. DNA-free™ DNA removal reagents are included in the kit, making the isolated RNA especially suitable for RT-PCR. This kit provides:
• Highly concentrated RNA—for increased sensitivity in downstream assays
• Isolation of high-quality RNA from micro-scale samples
• Reagents/protocol for RNA recovery from LCM samples
How it works
Samples are solubilized in a powerful chaotropic solution, and RNA is purified on a glass fiber filter cartridge specifically designed for very small samples. A simple modification of the protocol allows recovery of both large and small RNA species including tRNAs, 5S rRNA, and microRNAs. The RNA is eluted in a minimal volume of 20 µL. Residual genomic DNA is efficiently removed using the DNA-free™ system included in the kit. The RNAqueous®-Micro Kit contains enough reagents for 50 RNA isolations of DNA-free RNA.
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