规格
|
Sample Type:
|
Plasmid DNA |
Cell Type:
|
Established Cell Lines |
Classification:
|
Animal Origin-Free |
High-throughput Compatibility:
|
Not High-throughput Compatible (Manual) |
Serum Compatible:
|
Yes |
Shipping Condition:
|
Wet Ice |
Transfection Technique:
|
Lipid-Based Transfection |
Product Line:
|
Freestyle™ |
储存
|
Each unit contains one vial of reagent. Store at +4°C. FreeStyle&trade MAX is guaranteed stable for 6 months when stored properly. |
FreeStyle™ MAX Reagent is a proprietary, animal origin–free formulation for transfecting plasmid DNA into eukaryotic cells that can easily be scaled up to produce large amounts of recombinant proteins. FreeStyle™ MAX Reagent allows the highest expression levels and transfection rates with lowest cytotoxicity in bio-production applications, and is specifically formulated for use with:
• FreeStyle™ 293-F Cells (suspension human embryonal kidney cells, Cat. No. R790-07) in serum-free FreeStyle™ 293 Expression Medium (Cat. No. 12338-018)
• FreeStyle™ CHO-S Cells (suspension Chinese Hamster Ovary cells, Cat. No. R800-07) in serum-free FreeStyle™ CHO Expression Medium (Cat. No. 12651-014)
• DG44 Cells (DHFR– suspension CHO cells) in CD DG44 Medium with 8 mM L-glutamine and 18 mL/L 10% Pluronic® F-68 (Cat. No. A11000-01)
Product use
FreeStyle™ MAX Reagent is intended for use with the FreeStyle™ MAX 293 Expression System (Cat. No. K9000-10) and the FreeStyle™ MAX CHO Expression System (Cat. No. K9000-20).