GENOMIC DNA PURIFICATION KIT EA,K0512,赛默飞世尔

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订货号 : 2EX9152
品牌型号 : 赛默飞世尔 K0512
货期 : 询货期
最小订货量 : 1套
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产品介绍 Product Description
规格
For Use With (Application): Real-Time Quantitative PCR (qPCR), Cloning, Southern Blotting, Sequencing, PCR
Purification Target: Genomic DNA
Sample Type: Liquid Samples (e.g. Serum), Plant Samples, Cells, Tissue, Yeast, Bacteria, Blood
High-throughput Compatibility: Not High-throughput Compatible (Manual)
储存
• Lysis Solution, 40 mL
• Precipitation Solution (10X), 8 mL
• 1.2 M sodium chloride, 10 mL

Store at room temperature.
描述

Thermo Scientific Genomic DNA Purification Kit is a simple and rapid system for high quality genomic DNA purification from various sources including: whole blood, serum, cell lines, bacterial cells, plant, and mammalian tissues.

The kit is based on selective detergent-mediated DNA precipitation from crude lysate. The entire procedure is rapid only 20 to 25 minutes with a typical yield of 2 to 10 µg genomic DNA from 0.2 mL of blood.

High molecular weight genomic DNA purified with the kit is suitable for direct use in all common molecular biology applications: PCR, FastDigest and conventional restriction digestion, cloning, DNA sequencing, and Southern blot analysis.

Highlights

• Efficient—2 to 10 µg of genomic DNA from 0.2 mL of blood
• Fast—procedure takes approximately 20 minutes
• Universal—purifies genomic DNA from various sources
• Flexible—easy to scale up and down
• Safe—does not involve phenol extraction

Applications

• Purification of high-quality genomic DNA from:
• Whole blood or serum
• Cell culture
• Plant tissues
• Mammalian tissues
• Epithelium samples
• Bacteria Includes Lysis Solution Precipitation Solution NaCl Solution Detailed Protocol

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