- 商品介绍
- 规格参数
- 包装参数
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规格 |
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| Bacterial or Yeast Strain: | TOP10 |
| Blue/White Screening: | Yes |
| Cloning Methylated DNA: | Yes |
| Cloning Unstable DNA: | Not Suitable for cloning unstable DNA |
| Competent Cell Type: | Chemically Competent |
| F'episome (to make ssDNA): | Lacks F' Episome |
| High-throughput Compatibility: | Not High-throughput Compatible (Manual) |
| Plasmid: | High Copy Plasmid |
| Preparing Unmethylated DNA: | Not Suitable for preparing unmethylated DNA |
| Product Line: | One Shot™ |
| Propagating ccdB Vectors: | Not for ccdB vector propagation |
| Reduces Recombination: | Yes |
| Shipping Condition: | Dry Ice |
| T1 Phage - Resistant (tonA): | No |
| Transformation Efficiency: | > 1x10^9 |
| Transformation Efficiency Level: | High Efficiency (> 10^9 cfu⁄µg) |
| Format: | One Shot |
| Improves Plasmid Quality: | Yes |
| Species: | E. coli |
| Quantity: | 10 reactions |
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储存 |
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Contains: • One Shot® TOP10 Chemically Competent E. coli: 11 vials, 50 µl each • pUC19 DNA (10 pg/µl): 1 vial, 50 µl • S.O.C. medium: 1 bottle, 6 ml Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature. |
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描述 |
One Shot® TOP10 Chemically Competent E. coli are provided at a transformation efficiency of 1 x 109 cfu/µg plasmid DNA and are ideal for high-efficiency cloning and plasmid propagation. They allow stable replication of high-copy number plasmids and are the same competent cells that come with many of our cloning kits. One Shot® TOP10 cells:
• Maximize cloning efficiency in a single-tube format
• Provide enhanced genomic DNA cloning capabilities
Easy-to-use One Shot® format
The single-tube, single-use format allows for all steps of the transformation protocol, up to plating, to take place in the same tube, thereby helping to save time and to prevent contamination.
Versatile cloning capabilities
One Shot® TOP10 E. coli cells are similar to the DH10B™ strain, and offer the following features:
• hsdR for efficient transformation of unmethylated DNA from PCR amplifications
• mcrA for efficient transformation of methylated DNA from genomic preparations
• lacZΔM15 for blue/white color screening of recombinant clones
• endA1 for cleaner DNA preparations and better results in downstream applications due to elimination of nonspecific digestion by Endonuclease I
• recA1 for reduced occurrence of nonspecific recombination in cloned DNA
Genotype: F- mcrA Δ( mrr-hsdRMS-mcrBC) Φ80lacZΔM15 Δ lacX74 recA1 araD139 Δ( araleu)7697 galU galK rpsL (StrR) endA1 nupG
Find the strain and format that you need
We also offer many other different strains and formats of chemically competent cells and electrocompetent cells to help meet your specific transformation needs. If you require high-throughput transformation, choose from our collection of MultiShot™ formatted comp cells.
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| 重量(kg) |
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¥ 698.71
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¥ 32.20
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