规格
|
Form:
|
Liquid |
Mass:
|
9 A260 units |
Shipping Condition:
|
Approved for shipment on Wet or Dry Ice |
Primer:
|
Random Primers |
储存
|
100 µL of Random Primers are supplied at a concentration of 3 µg/µL in 3 mM Tris-HCl (pH 7.0), 0.2 mM EDTA. Store at -20°C. Guaranteed stable for 6 months when properly stored. |
Random Primers are oligodeoxyribonucleotides (mostly hexamers) used to prepare labeled DNA probes from templates for filter hybridization or in situ hybridization and to prime mRNAs with or without poly(A) for cDNA synthesis. These primers are truly random and are suitable for DNA synthesis using Klenow fragments with DNA templates or for cDNA synthesis using reverse transcriptase with mRNA templates. To avoid storage buffer interference, Random Primers are supplied in a low-salt-concentration buffer.
Performance and Quality Testing:
Incorporation of a radioactively labeled nucleotide is verified using control DNA in a Random Primers labeling reaction.