LC-MS/MS SUITABILITY STD 25UL，A40010，赛默飞世尔

The Pierce LC-MS/MS System Suitability Standard (7 x 5 mixture) contains seven HeavyPeptide AQUA Ultimate peptides provided at five dilutions distinguished by differential isotopic labeling to assess sensitivity and dynamic range of LC-MS/MS systems. The standard contains 7 of 15 Pierce Retention Time Calibration Mixture (Cat. No. 88320) peptides. Each of the seven peptides has five isotopologue versions containing no heavy, 1 heavy, 2 heavy, 3 heavy, and 4 heavy isotope-labeled amino acids. The isotopologues for each peptide are provided at a distinct concentrations to generate seven standard curves to assess system performance. The 7 x 5 mixture can be used with Skyline software (www.thermoscientific.com/pinpoint) to assess dynamic range, linearity, and lower limit of quantitation (LLOQ).

Pierce LC-MS/MS System Suitability Standard (7 x 5 mixture) features include:
• High quality—contains five sets of seven HeavyPeptide AQUA Ultimate peptides, with each peptide distinguishable through unique isotopologue labeling
• Optimized—enables scheduling of MS acquisition windows for improved quantification and increased multiplexing
• Quantitative—enables assessment of dynamic range of instrument and instrument sensitivity
• Predictive—enables assessment of chromatography and MS instrument performance

Applications:
• Standard for data-dependent acquisition (DDA)
• Standard for targeted MS (e.g., parallel-reaction monitoring (PRM))
• Optimization of LC parameters
• Identification of total peptide elution window
• Optimization of MS parameters
• Assessment dynamic range of nano or capillary flow LC-MS/MS systems

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EASY-Spray LC Analytical Column
Acclaim PepMap 100 C18 Trap Column, NanoViper
Pierce TMT11plex Yeast Digest Standard

General reference:
1. Krokhin OV, Spicer V. Peptide retention standards and hydrophobicity indexes in reversed-phase high-performance liquid chromatography of peptides. Anal Chem. 2009 81(22):9522-30.