PTK-GREEN RENILLA LUC, 0.5 UG/UL (10 UG),16154,赛默飞世尔

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订货号 8XH2342
品牌型号 赛默飞世尔 16154
货期 询货期
最小订货量 1套
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产品介绍 Product Description
规格
Cloning Method: Restriction Enzyme ⁄ MCS
Delivery Type: Transfection
Key Functions: Reporter Assays
Product Line: Pierce™
Promoter: TK
Reporter Gene: Green Renilla Luciferase
Selection Agent (Eukaryotic): Puromycin
Description: pTK-Green Renilla Luc Vector
储存
Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: -70 to -20°C.
描述

The Thermo Scientific pCMV-Green Renilla Luc vector is a derivative of pMCS-Green Renilla Luc. It contains the luciferase gene under the control of the Herpes Simplex Virus (HSV) thymidine kinase (TK) promoter, creating a constitutive expression vector that can be used as a normalization control to account for experimental variation in combination with other reporters.

Features of the pTK-Green Renilla Luc vector:

• Weak (TK) constitutive promoter for co-transfection and normalization
• Intracellular Green Renilla luciferase gene, optimized for high expression in mammalian systems
• Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimize background by reducing transcriptional read-through
• Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively
• High-copy pUC bacterial DNA replication origin

More Product Data
• Luciferase assays in hard-to-transfect Jurkat cells

Related Products
pMCS-Green Renilla Luc Vector for Luciferase Assays
pCMV-Green Renilla Luc Vector for Luciferase Assays

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