QIAamp DNA Blood Midi Kit (20),51183,Qiagen,凯杰

销售价: ¥ 3130.00 / 件
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订货号 8SE4441
品牌型号 Qiagen 51183
货期 45个工作日
最小订货量 1件
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产品介绍 Product Description

QIAamp DNA Blood Kits

For purification of genomic, mitochondrial or viral DNA from blood and other body fluids

Features

Rapid purification of high-quality, ready-to-use DNA

No organic extraction or alcohol precipitation

Consistent, high yields

Complete removal of contaminants and inhibitors for reliable results

Kit formats for low- to high-throughput – options for automation of all kits


产品特点 Features

QIAamp DNA Blood Kits provide silica-membrane-based DNA purification from whole blood, plasma, serum and other body fluids. The kits are designed for a range of sample sizes from 200 μl up to 10 ml fresh or frozen human whole blood. QIAamp spin columns can be easily processed in a centrifuge or on vacuum manifolds. A convenient 96-well format using centrifugation enables purification of DNA for labs that need high-throughput DNA purification from blood, buffy coat, plasma, serum, bone marrow, lymphocytes and body fluids. A dedicated kit is also available for automated purification of 1–12 samples on the QIAcube Connect.

QIAamp DNA Blood Kits yield DNA sized from 200 bp to 50 kb, depending on the age and storage of samples (see figure  "Apoptotic banding in stored blood"). The purified DNA is suitable for long-range PCR amplification (see figure " Long-range PCR") and restriction fragment length polymorphism (RFLP) analysis used, for example, for paternity testing (see figure " Paternity testing by RFLP analysis").

The QIAamp DNA Blood Midi Kit yields up to 94.5% recovery of DNA; the QIAamp DNA Blood Maxi Kit yields up to 95.8% recovery of DNA, depending on the starting cell densities (see table “DNA yields from human whole blood with different cell densities”).

DNA yields from human whole blood with different cell densities

Leukocytes per mlDNA yield (µg)DNA recovery (%)

QIAamp MidiQIAamp MaxiQIAamp MidiQIAamp Maxi
2.5 x 1053.015.892.395.8
1.0 x 10611.060.491.791.5
5.0 x 10662.4312.094.594.5
1.0 x 107116.3624.688.194.6


技术参数 Specifications

Comparison of QIAamp DNA Blood Kits

FeaturesQIAamp DNA Blood Mini KitQIAamp DNA Blood Midi KitQIAamp DNA Blood Maxi KitQIAamp DNA Blood Maxi Kit
ApplicationsPCR, long-range PCR, Southern blottingPCR, Southern blottingPCR, blottingSouthern blotting, genome mapping
Elution volume50–200 µl100–400 µl500–2000 µl50–200 µl
FormatSpin columnSpin columnSpin column96-well plate
Main sample typeWhole blood, body fluidsWhole blood, body fluidsWhole blood, body fluidsWhole blood, body fluids
ProcessingManual (centrifugation or vacuum)Manual (centrifugation or vacuum)Manual (centrifugation or vacuum)Manual (centrifugation or vacuum)
Purification of total RNA, miRNA, poly A+ mRNA, DNA or proteinGenomic DNA, mitochondrial DNA, viral DNAGenomic DNA, mitochondrial DNA, viral DNAGenomic DNA, mitochondrial DNA, viral DNAGenomic DNA, mitochondrial DNA, viral DNA
Sample amount1–200 µl0.3–2 ml3–10 ml<200 µl
TechnologySilica technologySilica technologySilica technologySilica technology
Time per run or per prep20–40 minutes55 minutes55 minutes2–3 hours (192 samples)
Yield4–12 µg20–60 µg300–600 µg6 µg


产品细节 Details

QIAamp DNA Blood Midi Kit (20),51183,Qiagen,凯杰

Paternity testing by RFLP analysis.

Three paternity cases tested with DNA purified from blood samples with the QIAamp DNA Blood Mini Kit. 1: mother; 2: child; 3: alleged father; 4: child + alleged father. M: markers. Each lane had 3 µg of DNA loaded. Outcome: A: alleged father excluded; B & C: alleged father confirmed.
(Data kindly provided by J. James, Gene Proof Technologies, Nashville, TN, USA.)

QIAamp DNA Blood Midi Kit (20),51183,Qiagen,凯杰

QIAamp Spin Column procedure.

Optimized buffers lyse samples, stabilize nucleic acids, and enhance selective DNA adsorption to the QIAamp membrane. Alcohol is added and lysates loaded onto the QIAamp spin column. Wash buffers are used to remove impurities and pure, ready-to-use DNA is then eluted in water or low-salt buffer.


产品应用 Applications

QIAamp DNA Blood Kits provide proven QIAamp technology for the purification of DNA from a variety of materials. Sample sources include:

  • Fresh and frozen whole blood or buffy coat

  • Plasma or serum

  • Bone marrow

  • Lymphocytes

  • Platelets

  • Body fluids

  • Cultured cells

  • Swabs and buccal cells


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