规格
|
Labeling Target:
|
Proteins (Glycoproteins) |
Label or Dye:
|
TAMRA Isomers, TMR (Tetramethylrhodamine) |
Product Line:
|
Click-iT™ |
Shipping Condition:
|
Room Temperature |
Detection Method:
|
Fluorescent |
储存
|
Store in freezer (-5 to -30°C). |
The Click-iT® Tetramethylrhodamine (TAMRA) Glycoprotein Detection Kit provides the second part of the simple and robust two-step technique to identify and characterize glycoproteins by one- or two-dimensional gel electrophoresis. In step two, after the incorporation of the azide handle into protein glycan structures with either a Click-iT® metabolic labeling reagent or the Click-iT® Enzymatic Labeling system, the azide-modified glycoproteins are detected via the chemoselective ligation or click reaction between an azide and an alkyne. Gels with TAMRA labeled glycoproteins can be subsequently stained with the Multiplexed Proteomics™ technologies, SYPRO® Ruby total protein stain and PRO-Q® Emerald glycoprotein stain for the differential analysis of glycoprotein subclasses, total proteins and total glycoproteins in the same gel. Click-iT® modified glycoproteins are also compatible with downstream LC-MS/MS and MALDI MS analysis for identification.