MEM ALPHA 10X1L，11900024，Invitrogen

规格
Form:	Powder
Glucose:	Low Glucose
Glutamine:	L-Glutamine
HEPES Buffer:	No HEPES
Phenol Red Indicator:	Phenol Red
Product Line:	Gibco®
Serum Supplementation:	Standard Serum Supplementation
Shipping Condition:	Room Temperature
Sodium Pyruvate Additive:	Sodium Pyruvate

储存

Storage conditions: 2-8° C Shipping conditions: Ambient Shelf life: 36 months from date of manufacture

描述

Minimum Essential Medium (MEM) α is widely used for mammalian cell culture as well as selection for transfected DHFR negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. We offer a variety of Gibco® MEM α modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

The complete formulation is available.

Gibco® MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B₁₂, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR negative cells. This product is made with Earle’s salts.

cGMP Manufacturing and Quality System
For supply chain continuity, we manufacture Gibco® MEM α at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.

MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM α uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH. Powder forms of Gibco® cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).