MEM EARLES 10x500ML，C11095500CP，Invitrogen

储存

Storage conditions: 2–8°C. Protect from light. Shipping conditions: ambient Shelf life: 12 months from date of manufacture

描述

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes.

MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow’s MEM, MEM α, DMEM, and Temin’s Modification. This MEM contains Earle’s salts for use in a CO₂ incubator. MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g ⁄ L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.

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