规格
|
Control Template:
|
Total RNA from Mouse Liver |
Format:
|
Tube(s) |
Gene Symbol:
|
ACTB |
Includes:
|
Control Primers & Template(s) |
Label or Dye:
|
Unconjugated |
Product Line:
|
Ambion™, QuantumRNA™ |
Shipping Condition:
|
Dry Ice |
Species:
|
Mouse |
Target Gene:
|
β-actin |
储存
|
β-Actin Primer Pair and β-Actin Competimer™s are stored at –20°C. Control Template RNA should be stored below –70°C. Nuclease-free water may be stored at any temperature. |
The Ambion® QuantumRNA™ β-actin Internal Standards with Competimer™ technology uses specially modified Competimer™s of the same sequence as the normal β-actin primers that cannot be extended. By adjusting the ratio of β-actin Competimer™s to normal β-actin mRNA primers, the signal for β-actin mRNA can be attenuated to the level of very rare messages. They are used for quantitative end-point RT-PCR and include sufficient reagents for 100 reactions. β-actin mRNA is an abundantly expressed "housekeeping" gene; therefore, it is difficult to multiplex primers for β-actin mRNA in the same PCR reaction with primers for rare messages. Typically the β-actin mRNA signal will overwhelm the relative RT-PCR reaction, and the rare gene-specific product goes undetected. β-actin Standards Product Size: 294 bp