规格
|
Assay Entry:
|
Biochemical binding/kinetic |
Kinase Group:
|
Serine/Threonine Kinase |
Species:
|
Human |
Target Entry:
|
CDK9, Inactive |
Molecular Weight:
|
69.1 kDa |
Gene Alias:
|
CDC2L4, C-2k, TAK, PITALRE |
Validated Application:
|
Kinase Assay |
Protein Tag:
|
GST Tag |
储存
|
Storage Buffer: 50 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.1 mM EGTA, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol
Store at -68 to -85°C. Aliquot at first use to avoid multiple freeze-thaws. |
CDK9 is a member of the cyclin-dependent protein kinase (CDK) family. CDK9 is closely related to cdc28 and cdc2 and is an important regulator of the cell cycle. CDK9 is a component of the multiprotein complex TAK/P-TEFβ. CDK9 can modulate RNA polymerase II-directed transcription by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. CDK9 forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. CDK9 also interacts with the HIV-1 Tat protein which suggested a possible involvement of this protein in AIDS. Recombinant full-length human CDK9 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
• Validated in LanthaScreen® Kinase Binding Assay
• Protocols available for simple use and optimized performance
• Multiple pack sizes available for convenient use
Related Product:
LanthaScreen® Eu Kinase Binding Assay