- 商品介绍
- 规格参数
- 包装参数
规格 |
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Concentration: | 200 U⁄µl |
Format: | Tube(s) |
Includes: | 1 × 1mL M-MLV RT (200U/μL); 1mL 5X First Strand Buffer [250mM Tris-HCl (pH 8.3), 375mM KCl, 15mM Magnesium Chloride]; 500μL 100mM DTT; 200,000 units |
No. of Reactions: | 1000 reactions |
Quantity: | 1000 reactions |
Sensitivity: | Low |
Enzyme Function: | RNA dependent DNA Polymerase |
Size (Final Product): | 7 kb or less |
Optimal Reaction Temperature: | 37° C |
Reverse Transcriptase: | M-MLV |
Ribonuclease H Activity: | Reduced |
Shipping Condition: | Wet Ice |
Template: | ssDNA, ssRNA, RNA-DNA Hybrid |
储存 |
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Contains: • 1 × 1 mL M-MLV RT (200 U/µl) • 1 ml 5X First Strand Buffer [250 mM Tris-HCl (pH 8.3), 375 mM KCl, 15 mM Magnesium Chloride] • 500 µl 100 mM DTT Store at -20°C. Guaranteed stable for 6 months when properly stored. |
描述 |
M-MLV Reverse Transcriptase is a recombinant DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. Compared to AMV RT, Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) lacks DNA endonuclease activity and has a lower RNase H activity. Features of this enzyme:
• Thermostability—optimal activity at 37°C
• Size of cDNA—M-MLV can be used to synthesize first-strand cDNA up to 7 kb
• Applications—synthesis of first-strand cDNA, primer extension, sequencing dsDNA, cDNA libraries, and RT-PCR
Source
Purified from E. coli expressing the pol gene of M-MLV on a plasmid
Performance and quality testing
SDS-PAGE purity; endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; and yield and length of cDNA product
Unit definition
One unit of M-MLV RT is the amount of enzyme required to incorporate 1 nmole of deoxyribonucleotide into acid-precipitable material in 10 min at 37°C using poly(A)oligo(dT)25 as template-primer.
Unit reaction conditions
50 mM Tris-HCl (pH 8.3), 40 mM KCl, 6 mM MgCl2, 1 mM DTT, 0.5 mM [3H]dTTP, 0.1 mM poly(A), 0.1 mM oligo(dT)25, 0.1 mg/mL BSA, and enzyme in 50 µL for 10 min at 37°C.
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