规格
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Classification:
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Protein-Free, Serum-Free |
Form:
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Liquid |
Glutamine:
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No Glutamine |
Product Line:
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Express Five®, Gibco® |
Serum Level:
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Serum-Free |
Shipping Condition:
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Room Temperature |
Cell Line:
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High Five™ |
Cell Type:
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Insect Cells |
储存
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Storage conditions: 2-8°C. Protect from light Shipping conditions: Ambient Shelf life: 18 months from date of manufacture |
Express Five® SFM is a protein-free, serum-free medium specifically developed to support the long-term suspension growth of High Five™ (BTI-TN-5B1-4) cells in suspension for use in the Baculovirus Expression Vector System (BEVS). This medium requires the addition of 16 mM L-glutamine before use. Gibco® Express Five® SFM features:
• Superior long-term suspension growth of High Five™ cells
• Protein-free, serum-free formulation
• Optimized for recombinant protein production
• Scalable in the CelliGen® bioreactor and WAVE Bioreactor® System
Superior growth of High Five™ cells
Gibco® Express Five® SFM provides peak cell densities of up to 6 × 106 viable cells per mL in shaker cultures. Cells adapted to other commercially available serum-free media can be subcultured directly into Gibco® Express Five® SFM, usually without any further adaptation (see figure). Cells usually require adaptation from serum-containing formulations.
Protein-free, serum-free formulation
Gibco® Express Five® SFM is a protein-free and serum-free formulation, allowing for easier purification of your protein of interest.
Optimized for recombinant protein expression
Gibco® Express Five® SFM is optimized for high protein production using the Baculovirus Expression Vector System (BEVS). Using BEVS, we routinely achieve rβ-galactosidase protein expression of up to 1.7 U/cell, a significant improvement over other media (see figure)
Scalable in the CelliGen® bioreactor and WAVE Bioreactor® System
Protein production using Gibco® Express Five® SFM can be scaled up in bioreactors such as the 5 L CelliGen® bioreactor and the WAVE Bioreactor® System. The appropriate impeller or rocking speed and seeding density should be optimized for each system. Depending on the bioreactor design, it may be necessary to supplement Gibco® Express Five® SFM with additional Pluronic® F-68 to avoid sheer stress in the culture.
cGMP manufacturing and quality system
Gibco® Express Five® SFM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.