PepTivator Dengue 02 Protein C – RG，130-123-131，美天旎

The PepTivator^® Dengue Virus Type 2 Capsid Protein C is a pool of lyophilized peptides, consisting mainly of 15-mer sequences with 11 amino acids overlap, covering the sequence of the Dengue Virus Type 2 Capsid Protein C. The sequence is derived from UniProt Acc. no. P14340 and covers aa 1–117 of the genome polyprotein.

In vitro stimulation of antigen-specific T cells with PepTivator Peptide Pools causes the secretion of effector cytokines and the up-regulation of activation markers, which then allow the detection and isolation of antigen-specific T cells.

The Dengue virus can be found in tropical and subtropical areas worldwide and belongs to the family of flaviviruses. Dengue fever is a mosquito transmitted viral infection and can be caused by four distinct serotypes (DEN-1, DEN-2, DEN-3, and DEN-4), inducing high fever, severe muscle pain, and nausea. Dengue virus type 2 (DENV-2) is the most common infecting serotype. Recovery from infection by one Dengue virus induces immunity against the homologous serotype but not against the other three serotypes. Secondary infection with a different DENV serotype can cause the potentially life-threatening dengue haemorrhagic fever (DHF) and dengue shock syndrome (DSS).

PepTivator^® Dengue Virus Type 2 Capsid Protein C – research grade has been specifically developed for efficient in vitro stimulation of Dengue–specific T cells. Peptides of 15 amino acids in length and 11 amino acids overlap represent an optimized solution for stimulating both CD4⁺ and CD8⁺ T cells in various applications, including:

• Detection and analysis of antigen-specific CD4⁺ and CD8⁺ effector/memory T cells in PBMCs by MACS^® Cytokine Secretion Assays, intracellular cytokine staining, or other technologies.
• Isolation of viable antigen-specific CD4⁺ T cells with the CD154 MicroBead Kit, or of CD4⁺ and CD8⁺ T cells using the CD137 MicroBead Kit or MACS Cytokine Secretion Assay – Cell Enrichment and Detection Kits. Subsequently, cells can be expanded for generation of T cell lines.
• Generation of antigen-specific effector/memory T cells from naive T cell populations.
• Pulsing of antigen-presenting cells.